Research Article Open Access

Effect of Repeated Freezing and Thawing on Nguni Sperm Parameters Evaluated by Computer Assisted Sperm Analyzer System

Masindi Lottus Mphaphathi1 and Tshimangadzo Lucky Nedambale2
  • 1 University of the Free State, South Africa
  • 2 Tshwane University of Technology, South Africa

Abstract

The aims of the current study were to assess the effect of double freezing and thawing on Nguni semen parameters. Semen was collected from Nguni bulls. The Sperm Class Analyzer® system was used to evaluate sperm motility rates prior and post freezing, repeatedly. Semen samples were diluted with egg yolk citrate extender. Cooled semen samples were loaded into straws and placed inside the programmable freezer for freezing. The frozen semen straws were stored inside liquid nitrogen tank. The frozen semen straws were then thawed at 15°C (1st and 2nd freezing and thawing followed the same process) for a duration of 5 min and further assessed post-thawed at 0 and 15 min during in vitro liquid storage at 15°C. The treatment means were analyzed using Fisher’s protected t-test least. There was no significant differences recorded on raw semen sperm total motility (93.2%) and 1st frozen-thawed at 0 min (82.6%), with recovery rate of 88.6% of the sperm total motility. There was a drastic decline observed in sperm total motility during the 1st frozen-thaw at 15 min (77.6%), 2nd frozen-thaw at 0 min (31.3%) and 2nd frozen-thaw at 15 min (30.1%; P<0.05). In conclusion, the freezing and thawing process did not decrease the bull’s sperm total motility percentage during the 1st freezing and thawing process, as compared with raw semen. However, a drastic decline was observed during the 2nd freezing-thawing process, as compared with raw semen.

American Journal of Animal and Veterinary Sciences
Volume 16 No. 1, 2021, 1-6

DOI: https://doi.org/10.3844/ajavsp.2021.1.6

Submitted On: 26 August 2020 Published On: 27 January 2021

How to Cite: Mphaphathi, M. L. & Nedambale, T. L. (2021). Effect of Repeated Freezing and Thawing on Nguni Sperm Parameters Evaluated by Computer Assisted Sperm Analyzer System. American Journal of Animal and Veterinary Sciences, 16(1), 1-6. https://doi.org/10.3844/ajavsp.2021.1.6

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Keywords

  • Bulls
  • Semen
  • Cryopreservation