Research Article Open Access

The Motility and Ratio of X and Y Sperm Filial Ongole Cattle Using Different Sexed Semen Methods

Enike Dwi Kusumawati1, N. Isnaini2, A.P.A. Yekti2, M. Luthfi3, L. Affandhy3, D. Pamungkas3, Kuswati2, A. Ridhowi2, H. Sudarwati2, Syam Rahadi4, S. Rahayu2 and Trinil Susilawati2
  • 1 Universitas Kanjuruhan Malang, Indonesia
  • 2 Brawijaya University, Indonesia
  • 3 Beef Cattle Research Station, Indonesia
  • 4 Universitas Halu Oleo, Indonesia

Abstract

Artificial Insemination (AI) using sexed semen produce sex of calf as expected. There are various methods of sexed semen has been found. The aim of this research is to determine the effect of sexed semen methods using egg white sedimentation and percoll density gradient centrifugation toward motility and ratio of X and Y sperm Filial Ongole Cattle. Semen was collected using Artificial Vagina (AV) from Filial Ongole Cattle, evaluated and then separated using egg white sedimentation and percoll density gradient centrifugation technique. Only fresh semen with a minimum of 70% individual motile sperm and 2+ mass motility used in this study. Andromed as a based extender was diluted using aquabidest with 1:4 ratio. The obtained data were analyzed with analysis of variance (ANOVA) and continued by Duncan test if there was significant or very significant different. The result showed that the sexing methods (non sexing sperm, upper and under fraction using percoll density gradient centrifugation, upper and under fraction using egg white sedimentation technique) had very significant effect (P<0.01) on motility (64.25±3.94%; 48.55±8.28%; 53±7.93%; 56.9±8.22%; 49.75±8.19% respectively) and significant effect (P<0.05) on the ratio of X and Y sperm. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation methods for the upper fraction were 28.0±2.26%:72.0±2.26% and 77.5±1.26%:22.5±1.26%. The ratio of X and Y using percoll density gradient centrifugation and egg white sedimentation technique for the under fraction were 69.0±15.35%: 26.0±4.37% and 22.9±1.44%: 77.1±1.44%. The study concludes that the sexed with egg white sedimentation technique is better than percoll density gradient centrifugation on sperm motility and ratio of X and Y sperm.

American Journal of Animal and Veterinary Sciences
Volume 14 No. 2, 2019, 111-114

DOI: https://doi.org/10.3844/ajavsp.2019.111.114

Submitted On: 22 March 2018 Published On: 9 May 2019

How to Cite: Kusumawati, E. D., Isnaini, N., Yekti, A., Luthfi, M., Affandhy, L., Pamungkas, D., Kuswati, Ridhowi, A., Sudarwati, H., Rahadi, S., Rahayu, S. & Susilawati, T. (2019). The Motility and Ratio of X and Y Sperm Filial Ongole Cattle Using Different Sexed Semen Methods. American Journal of Animal and Veterinary Sciences, 14(2), 111-114. https://doi.org/10.3844/ajavsp.2019.111.114

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Keywords

  • Separation
  • Methods
  • Sexed
  • Sperm Quality
  • Sperm Proportion