@article {10.3844/ajavsp.2019.111.114,
article_type = {journal},
title = {The Motility and Ratio of X and Y Sperm Filial Ongole Cattle Using Different Sexed Semen Methods},
author = {Kusumawati, Enike Dwi and Isnaini, N. and Yekti, A.P.A. and Luthfi, M. and Affandhy, L. and Pamungkas, D. and Kuswati, and Ridhowi, A. and Sudarwati, H. and Rahadi, Syam and Rahayu, S. and Susilawati, Trinil},
volume = {14},
number = {2},
year = {2019},
month = {May},
pages = {111-114},
doi = {10.3844/ajavsp.2019.111.114},
url = {https://thescipub.com/abstract/ajavsp.2019.111.114},
abstract = {Artificial Insemination (AI) using sexed semen produce sex of calf as expected. There are various methods of sexed semen has been found. The aim of this research is to determine the effect of sexed semen methods using egg white sedimentation and percoll density gradient centrifugation toward motility and ratio of X and Y sperm Filial Ongole Cattle. Semen was collected using Artificial Vagina (AV) from Filial Ongole Cattle, evaluated and then separated using egg white sedimentation and percoll density gradient centrifugation technique. Only fresh semen with a minimum of 70% individual motile sperm and 2+ mass motility used in this study. Andromed as a based extender was diluted using aquabidest with 1:4 ratio. The obtained data were analyzed with analysis of variance (ANOVA) and continued by Duncan test if there was significant or very significant different. The result showed that the sexing methods (non sexing sperm, upper and under fraction using percoll density gradient centrifugation, upper and under fraction using egg white sedimentation technique) had very significant effect (P},
journal = {American Journal of Animal and Veterinary Sciences},
publisher = {Science Publications}
}