Research Article Open Access

Multidrug-Resistant Bacterial Pathogens Assessment in Canine Ophthalmic Infections

Ricardo Antonio Pilegi Sfaciotte1, Lincoln Garcia Coronel2, Alessandra Snak3, Jéssica Tainá Bordin4, Leandro Kiyoshi Yamamoto5, Vanessa Kelly Capoia Vignoto4, Sílvia Cristina Osaki3 and Sheila Rezler Wosiacki4
  • 1 Santa Catarina State University, Brazil
  • 2 Federal University of Santa Catarina, Brazil
  • 3 Federal University of Parana, Brazil
  • 4 State University of Maringa, Brazil
  • 5 Qualittas Institute, Brazil

Abstract

The objective of this study was to identify the main microorganisms associated with ophthalmic infections and determine the resistance profile of these isolates against antimicrobial drugs. 26 bacterial isolates from 18 canine ophthalmic infections were submited to the phenotypic resistance profile for 36 drugs of 12 classes of antimicrobials, research of multidrug-resistant strains with importance in public health and detection of Staphylococcus mecA gene by PCR. The bacterial isolates were identified as Staphylococcus spp. (n = 18), Enterococcus spp. (n = 1), enterobacteria (n = 6) and Pseudomonas spp. (n = 1). The percentage of resistance and intermediate resistance were 42.48% (n = 325). Considering separate antimicrobials drugs, 18 isolates were characterized by multidrug resistant, while by the assesment of resistance to class, 20 isolates were multiresistant. In the phenotypic detection, 61.11% (11/18) of Staphylococcus spp. were predicted by Methicillin-Resistant Staphyloccus (MRS), whereas the genotypic detection, 38.89% (7/18) were carriers of the mecA gene. Two enterobacterias were considered producers of expectro Extended of Betalactamase (ESBL). EUCAST was more reliable for detecting MRS strains than the CLSI. The present study detected multiresistant isolates of great importance and are involved in cases of public health, such as MRS, MRSMLSb, ESBL, very important to be readily identified and controled so as to prevent the spread of this type of resistance.

American Journal of Animal and Veterinary Sciences
Volume 13 No. 1, 2018, 7-15

DOI: https://doi.org/10.3844/ajavsp.2018.7.15

Submitted On: 21 July 2017 Published On: 2 January 2018

How to Cite: Pilegi Sfaciotte, R. A., Coronel, L. G., Snak, A., Bordin, J. T., Yamamoto, L. K., Capoia Vignoto, V. K., Osaki, S. C. & Wosiacki, S. R. (2018). Multidrug-Resistant Bacterial Pathogens Assessment in Canine Ophthalmic Infections. American Journal of Animal and Veterinary Sciences, 13(1), 7-15. https://doi.org/10.3844/ajavsp.2018.7.15

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Keywords

  • Multiresistant
  • MRS
  • mecA
  • ESBL
  • Public Health