TY - JOUR AU - Pilegi Sfaciotte, Ricardo Antonio AU - Coronel, Lincoln Garcia AU - Snak, Alessandra AU - Bordin, Jéssica Tainá AU - Yamamoto, Leandro Kiyoshi AU - Capoia Vignoto, Vanessa Kelly AU - Osaki, Sílvia Cristina AU - Wosiacki, Sheila Rezler PY - 2018 TI - Multidrug-Resistant Bacterial Pathogens Assessment in Canine Ophthalmic Infections JF - American Journal of Animal and Veterinary Sciences VL - 13 IS - 1 DO - 10.3844/ajavsp.2018.7.15 UR - https://thescipub.com/abstract/ajavsp.2018.7.15 AB - The objective of this study was to identify the main microorganisms associated with ophthalmic infections and determine the resistance profile of these isolates against antimicrobial drugs. 26 bacterial isolates from 18 canine ophthalmic infections were submited to the phenotypic resistance profile for 36 drugs of 12 classes of antimicrobials, research of multidrug-resistant strains with importance in public health and detection of Staphylococcus mecA gene by PCR. The bacterial isolates were identified as Staphylococcus spp. (n = 18), Enterococcus spp. (n = 1), enterobacteria (n = 6) and Pseudomonas spp. (n = 1). The percentage of resistance and intermediate resistance were 42.48% (n = 325). Considering separate antimicrobials drugs, 18 isolates were characterized by multidrug resistant, while by the assesment of resistance to class, 20 isolates were multiresistant. In the phenotypic detection, 61.11% (11/18) of Staphylococcus spp. were predicted by Methicillin-Resistant Staphyloccus (MRS), whereas the genotypic detection, 38.89% (7/18) were carriers of the mecA gene. Two enterobacterias were considered producers of expectro Extended of Betalactamase (ESBL). EUCAST was more reliable for detecting MRS strains than the CLSI. The present study detected multiresistant isolates of great importance and are involved in cases of public health, such as MRS, MRSMLSb, ESBL, very important to be readily identified and controled so as to prevent the spread of this type of resistance.