Research Article Open Access

Production of (E) 10-Hydroxy-8-octadecenoic Acid with Lyophilized Microbial Cells

A. Bódalo1, J. Bastida1, M.F. Máximo1, A. M. Hidalgo1 and M. D. Murcia1
  • 1 University of Murcia, Spain

Abstract

Lyophilized non-proliferating microbial cells of Pseudomonas sp. 42A2 showed their ability to catalyze the bioconversion of oleic acid into (E)-10-hydroxy-8-octadecenoic acid (MHOD) and (E)- 7,10-dihydroxy-8-octadecenoic acid (DHOD). The reaction conditions were optimized in order to produce the maximum amount of MHOD. At 30°C and using phosphate buffer (0.1 M and pH=8), an initial substrate concentration of 10 mg mL-1 and a cell concentration of 8 mg mL-1, practically all the oleic acid in the medium was biotransformed within 42 h, with hardly any DHOD produced (1.4 mg mL-1).

American Journal of Biochemistry and Biotechnology
Volume 1 No. 1, 2005, 1-4

DOI: https://doi.org/10.3844/ajbbsp.2005.1.4

Submitted On: 23 February 2005 Published On: 31 March 2005

How to Cite: Bódalo, A., Bastida, J., Máximo, M., Hidalgo, A. M. & Murcia, M. D. (2005). Production of (E) 10-Hydroxy-8-octadecenoic Acid with Lyophilized Microbial Cells. American Journal of Biochemistry and Biotechnology, 1(1), 1-4. https://doi.org/10.3844/ajbbsp.2005.1.4

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Keywords

  • Hydroxy Fatty Acids
  • Pseudomonas sp.
  • Biotransformation
  • Oleic Acid