TY  - JOUR
AU  - Bódalo, A. 
AU  - Bastida, J. 
AU  - Máximo, M.F. 
AU  - Hidalgo, A. M. 
AU  - Murcia, M. D. 
PY  - 2005
TI  - Production of (E) 10-Hydroxy-8-octadecenoic Acid with Lyophilized Microbial Cells
JF  - American Journal of Biochemistry and Biotechnology
VL  - 1
IS  - 1
DO  - 10.3844/ajbbsp.2005.1.4
UR  - https://thescipub.com/abstract/ajbbsp.2005.1.4
AB  - Lyophilized non-proliferating microbial cells of Pseudomonas sp. 42A2 showed their ability
to catalyze the bioconversion of oleic acid into (E)-10-hydroxy-8-octadecenoic acid (MHOD) and (E)-
7,10-dihydroxy-8-octadecenoic acid (DHOD). The reaction conditions were optimized in order to
produce the maximum amount of MHOD. At 30°C and using phosphate buffer (0.1 M and pH=8), an
initial substrate concentration of 10 mg mL-1 and a cell concentration of 8 mg mL-1,
practically all the oleic acid in the medium was biotransformed within 42 h, with hardly any DHOD
produced (1.4 mg mL-1).