@article {10.3844/ajbbsp.2005.1.4, article_type = {journal}, title = {Production of (E) 10-Hydroxy-8-octadecenoic Acid with Lyophilized Microbial Cells}, author = {Bódalo, A. and Bastida, J. and Máximo, M.F. and Hidalgo, A. M. and Murcia, M. D.}, volume = {1}, number = {1}, year = {2005}, month = {Mar}, pages = {1-4}, doi = {10.3844/ajbbsp.2005.1.4}, url = {https://thescipub.com/abstract/ajbbsp.2005.1.4}, abstract = {Lyophilized non-proliferating microbial cells of Pseudomonas sp. 42A2 showed their ability to catalyze the bioconversion of oleic acid into (E)-10-hydroxy-8-octadecenoic acid (MHOD) and (E)- 7,10-dihydroxy-8-octadecenoic acid (DHOD). The reaction conditions were optimized in order to produce the maximum amount of MHOD. At 30°C and using phosphate buffer (0.1 M and pH=8), an initial substrate concentration of 10 mg mL-1 and a cell concentration of 8 mg mL-1, practically all the oleic acid in the medium was biotransformed within 42 h, with hardly any DHOD produced (1.4 mg mL-1).}, journal = {American Journal of Biochemistry and Biotechnology}, publisher = {Science Publications} }