Adipose-Derived Canine Mesenchymal Stem Cells in PLAY® Media

Abstract

In veterinary medicine, stem cell-based therapies present promise for addressing orthopedic and degenerative conditions in canines. Mesenchymal Stem Cells (MSCs) possess unique regenerative, immunomodulatory, and differentiation capabilities. However, their efficacy relies on optimal in-vitro conditions. Prolonged expansion over passages leads to a gradual loss of stem cell traits, highlighting the importance of optimized culture conditions and media supplements. This study focuses on optimizing culture conditions for adipose-derived canine MSCs using play®, an in-house product containing growth factors and bioactive modulators. Our study provides insights into cellular properties, growth kinetics, and differentiation ability aiming to enhance adipose-derived canine MSCs' therapeutic potential in the field of veterinary medicine. Adipose-derived canine MSCs (AD-cMSCs) were isolated from adipose tissue from a canine source with veterinary professionals at Cessna Lifeline Veterinary Hospital, with prior pet owner consent. AD-cMSCs were cultured in 10% PLAY® or 10% Fetal Bovien Serum-supplemented media. Population Doubling Time (PDT) assessed growth rates. Trilineage differentiation potential was evaluated with specific media and morphology was examined under microscopy. Pluripotency gene expression was performed using a semi-quantitative polymerase chain reaction. AD-cMSCs cultured with 10% play® showed accelerated growth, enhanced differentiation potential, and elevated pluripotency marker expression compared to those in 10% FBS. These findings underscore the efficacy of play® as a promising culture supplement for optimizing AD-cMSC expansion and advancing their therapeutic potential in veterinary regenerative medicine