Research Article Open Access

The Success of Double Dose Artificial Insemination at Different Times in Ongole Crossbred Cattle

Aulia Puspita Anugra Yekti1, Rizki Prafitri1, Kuswati1, Asri Nurul Huda1, Kusmartono1 and Trinil Susilawati1
  • 1 Universitas Brawijaya, Indonesia


Artificial insemination technology has been proved to increase cattle productivity. This research aims to evaluate the success rate of double-dose artificial insemination at different times in Ongole crossbred cattle. This research was done in cattle household farms in Senggreng Village, Sumber Pucung District, Malang Regency, East Java, Indonesia. Fifty cattle involved randomly in this research met the criteria; Ongole crossbred, 4-11 years old, minimum Body Condition Score 3 points (1-9 points scale), reproductive sound, have given birth. Twenty-five cattle (T1) were inseminated 2 and 8 h after the estrus sign, while the other 25 (T2) were inseminated 8 h and 16 h after the estrus sign. After the first insemination, the cattle were injected with vitamin Bio ATP 10 mL and 3 kg concentrate feed (protein 12%) for three days. The next estrus sign was observed for the first and second cycles (NRR1 and NRR2). Re-insemination was done to cattle with an estrus sign-on NRR1. Palpation per rectal and ultrasonography was performed to determine the conception rate, pregnancy rate and services per conception. The study showed that double-dose artificial insemination at 2 and 8 h (T1) had better results than T2 (8 and 16 h). NRR1 for T1 = 84 and T2 = 76%; NRR2 for T1 = 80 and T2 = 76%; Conception Rate of T1 = 44% and T2 = 32%; Pregnancy Rate of T1 = 64 and T2 = 52%; services per conception of T1 = 1,32 and T2 = 1,24. In conclusion, double dose artificial insemination given at the 2nd and 8th h after estrus sign had better result on pregnancy than double dose insemination given at the 8th and 16th h.

American Journal of Animal and Veterinary Sciences
Volume 17 No. 1, 2022, 26-30


Submitted On: 23 August 2021 Published On: 10 February 2022

How to Cite: Yekti, A. P. A., Prafitri, R., Kuswati, ., Huda, A. N., Kusmartono, . & Susilawati, T. (2022). The Success of Double Dose Artificial Insemination at Different Times in Ongole Crossbred Cattle. American Journal of Animal and Veterinary Sciences, 17(1), 26-30.

  • 2 Citations



  • Ongole Crossbred
  • Artificial Insemination
  • Double Dose
  • Conception rate
  • Non-Return Rate