Research Article Open Access

Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria

Sabrina Rabehi1, Bakir Mamache1, Taha Mossadak Hamdi2, Asma Meghezzi3 and Khaoula Boushaba3
  • 1 University of Batna 1, Batna, Algeria
  • 2 High National Veterinary School, Algiers, Algeria
  • 3 Laboratory of Molecular Biology and Microbiology of Constantine Biotechnology Research Center, Algeria

Abstract

The aim of this study is the detection of the presence of L. monocytogenes in raw milk in the region of Batna using the real-time polymerase chain reaction (real-time PCR) technique, in order to assess the riskrun by the consumer of raw milk in the study area. 65 milk samples were investigated for the detection of L. monocytogenes. The detection of the hlyA gene in all samples was done by real-time PCR assay by comparative cycle threshold method. The results obtained were negative for all the samples analyzed, therefore the prevalence of L. monocytogenes in the 65 samples collected in this study is 0%. The absence of L. monocytogenes in the samples could be linked to the good health of the selected cattle. In fact, L. monocytogenes is considered to be very widespread in industrialized countries and rare in North Africa and Algeria.

American Journal of Animal and Veterinary Sciences
Volume 16 No. 4, 2021, 212-216

DOI: https://doi.org/10.3844/ajavsp.2021.212.216

Submitted On: 3 August 2021 Published On: 15 October 2021

How to Cite: Rabehi, S., Mamache, B., Hamdi, T. M., Meghezzi, A. & Boushaba, K. (2021). Prevalence of Listeria monocytogenes in Raw Cow's Milk in the Region of Batna, Algeria. American Journal of Animal and Veterinary Sciences, 16(4), 212-216. https://doi.org/10.3844/ajavsp.2021.212.216

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Keywords

  • Listeria monocytogenes
  • Real-Time PCR
  • Raw Milk
  • Public Health