Research Article Open Access

Optimization of Conditions for Extraction of Pepsin-Soluble Collagen from Indonesian Local “Kacang” Goatskin by Response Surface Methodology

Rina Wahyuningsih1, Rusman1, Nurliyani1, Ambar Pertiwiningrum1, Abdul Rohman1, Nanung Agus Fitriyanto1 and Yuny Erwanto1
  • 1 University of Gadjah Mada, Indonesia

Abstract

Indonesian local “Kacang” goat is an animal species widely known by consumers over the world which possesses many health effects. Goatskin is one of body parts which has potential as collagen sources. The optimal conditions for collagen extraction were determined by response surface methodology in this study. Multiple response optimization designs were applied to evaluate the effects of two independent variables (curing time of NaOH and hydrolysis time) on the pepsin-soluble collagen yield of Indonesian local “Kacang” goatskin. Correlation analysis of the mathematical regression model indicated that quadratic polynomial model could be employed to optimize the extraction of pepsin-soluble collagen. The optimal conditions to obtain the highest yield of pepsin-soluble collagen was without curing NaOH and 24 h time of hydrolysis.

American Journal of Animal and Veterinary Sciences
Volume 13 No. 2, 2018, 70-75

DOI: https://doi.org/10.3844/ajavsp.2018.70.75

Submitted On: 10 March 2018 Published On: 12 May 2018

How to Cite: Wahyuningsih, R., Rusman, Nurliyani, Pertiwiningrum, A., Rohman, A., Fitriyanto, N. A. & Erwanto, Y. (2018). Optimization of Conditions for Extraction of Pepsin-Soluble Collagen from Indonesian Local “Kacang” Goatskin by Response Surface Methodology. American Journal of Animal and Veterinary Sciences, 13(2), 70-75. https://doi.org/10.3844/ajavsp.2018.70.75

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Keywords

  • Indonesia Local “Kacang” Goatskin
  • Pepsin-Soluble Collagen
  • Extraction
  • Response Surface Methodology