Research Article Open Access

Kinetic Motilities of Cryopreserved Bull Spermatozoa: Owing to the Effect of Eurycoma longifolia Jack Aqueous Extract

Falah Hasan Baiee1, Abd Wahid Haron1, Rosnina Haji Yusoff1, Mohamed Ariff Omar1, Nurhusien Yimer1, Zaid Jeber1, Salman Hammadi1, Tarig Aldelrouf Ahmedeltayeb1, Wan-Nor Fitri1 and Muhammad Umar2
  • 1 Universiyi Putra Malaysia, Malaysia
  • 2 University of Agriculture, Pakistan

Abstract

This study was carried out to improve the kinetic motilities of frozen-thawed bull semen diluted with tris-based egg yolk diluent that was supplemented with Eurycoma longifolia jack aqueous extract. A total of 24 ejaculates were obtained from six cross-bred bulls using an electro-ejaculator. The extract of Eurycoma longifolia jack was distributed into three low doses and three high doses; cryopreserved samples were evaluated into three different times to confirm the results of kinetic motilities through different times and between groups. Path velocity (VAP μm/s), progress velocity (VSL μm/s), track speed (VCL μm/s), lateral amplitude (ALH μm/s), Beat Frequency (BCF Hz), straightness (STR %), linearity (LIN %), were evaluated three different times using Computer-assisted sperm analysis. Results revealed that the percentage of VAP, ASL and VCL were higher (p<0.05) in the frozen-thawed semen group supplemented with 5 mg mL-1 Eurycoma longifolia jack extract (73.19±1.91, 58.34±2.06 and 117.91±2.68 in first evaluation, then 74.22±2.06, 57.45±1.72 and 118.92±2.55 in second evaluation and 72.95±2.27, 56.75±1.30 and 119.07±3.54 in third evaluation; respectively). In conclusion, Eurycoma longifolia Jack aqueous extract supplementation to the semen diluent at 5 mg mL-1 significantly improved sperm kinetic motilities of frozen-thawed bull semen.

American Journal of Animal and Veterinary Sciences
Volume 12 No. 2, 2017, 77-84

DOI: https://doi.org/10.3844/ajavsp.2017.77.84

Submitted On: 31 March 2017 Published On: 16 June 2017

How to Cite: Baiee, F. H., Haron, A. W., Yusoff, R. H., Omar, M. A., Yimer, N., Jeber, Z., Hammadi, S., Ahmedeltayeb, T. A., Fitri, W. & Umar, M. (2017). Kinetic Motilities of Cryopreserved Bull Spermatozoa: Owing to the Effect of Eurycoma longifolia Jack Aqueous Extract. American Journal of Animal and Veterinary Sciences, 12(2), 77-84. https://doi.org/10.3844/ajavsp.2017.77.84

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Keywords

  • Bull Semen
  • Kinetic Motility
  • Cryopreservation
  • Eurycoma longifolia
  • Tongkat Ali