Research Article Open Access

Development of a Short-Term Canine Full-Thickness Skin Organ Culture Method under Serum-Free Conditions

Francesca Abramo1, Andrea Pirone1, Carla Lenzi1, Maria Federica della Valle2, Silvia Vidali3, Iacopo Vannozzi1 and Vincenzo Miragliotta1
  • 1 University of Pisa, Italy
  • 2 Science Information and Documentation Centre (CeDIS), Italy
  • 3 University of Luebeck, Germany

Abstract

Full-thickness canine skin organ culture models could provide an entirely new opportunity for studying wound healing, keratinization disorders and allergic skin diseases, all of which have high prevalence and severe impact on canine quality of life. Here we present a canine organ culture method for the short-term maintenance of full-thickness, adult, canine skin in serum-free medium and investigate the possibility to induce mast cell degranulation ex vivo. General morphological features were maintained up to day 7. Epidermal thickness started to decrease from day 4 of culture. No changes were observed in epidermal pigmentation. Keratinocyte proliferation started to significantly decrease at day 7. Immunostaining for cytokeratin 10, cytokeratin 14 and loricrin was evident from day 1 to day 7. Compound 48/80 induced mast cell degranulation. This was the first attempt to establish a dog skin organ culture and document good preservation of most cutaneous structures till day 7. This method may help in dissecting canine skin biology in physiological and pathological conditions and to study drug mechanism of action in a biologically relevant environment.

American Journal of Animal and Veterinary Sciences
Volume 11 No. 2, 2016, 61-69

DOI: https://doi.org/10.3844/ajavsp.2016.61.69

Submitted On: 23 March 2016 Published On: 13 June 2016

How to Cite: Abramo, F., Pirone, A., Lenzi, C., della Valle, M. F., Vidali, S., Vannozzi, I. & Miragliotta, V. (2016). Development of a Short-Term Canine Full-Thickness Skin Organ Culture Method under Serum-Free Conditions. American Journal of Animal and Veterinary Sciences, 11(2), 61-69. https://doi.org/10.3844/ajavsp.2016.61.69

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Keywords

  • Organ Culture
  • Skin
  • Dog
  • Histology