Research Article Open Access

HISTOPATHOLOGICAL CHANGES IN MICE INFECTED WITH RIVER WATER CONTAMINATED BY PASTEURELLA MULTOCIDA TYPE B:2

Mohammed Muqdad Khaleel1, Faez Firdaus Jesse Abdullah2, Lawan Adamu3, Yusuf Abba3, Abdul Wahid Haron2, Mohd Zamri Saad2 and Abdul Rahman Omar4
  • 1 University of Mosul, Iraq
  • 2 Universiti Putra Malaysia, Malaysia
  • 3 University of Maiduguri, Nigeria
  • 4 University of Maiduguri, Malaysia

Abstract

Haemorrhagic Septicaemia (HS) is a fatal systemic disease of bovines typified by an acute, highly fatal septicaemic disease with high morbidity and mortality. In this study, sixty five adult ICR mice were used for the study. In the first phase, 5 mice each were inoculated with 109 cfu of P. multocida type B:2 through the intraperitoneal route. After development of classical signs of HS, the mice were euthanised and dropped into a tank of river waterfor 24, 48 and 72 h. Contaminated water from the tank were collected at the three different times (24, 48 and 72 h) and was used for intraperitoneal, oral and aerosol inoculation of 15 mice each with a dose of 1 mL-1 109 cfu (intraperitoneal and aerosol) and 0.4 mL-1 109cfu (oral). The control group (n = 5) was given phosphate buffered saline. All mice in the three groups and control were culled after 72 h post inoculation and the vital organs (liver, spleen, lung, kidney, heart, brain) were collected in 10% buffered formalin, procesed, sectioned and stained with H&E. Degeneration and necrosis, hemorrhage and infiltration of inflammatory cells were the most observed lesions in all the organs, while thrombosis and oedema were least observed. The lesion severity was moderate to severe using contaminated water at 72 h post contamination and mild to moderate when inoculated with water 24 and 48 h post contamination. Mice inoculated through the oral and intraperitoneal routes recorded more severe lesion compared with those inoculated through the aerosol route. However, using contaminated water at 24 h, mice inoculated through the aerosol route had the highest distribution of inflammatory cells in the lungs, which declined following inoculation with contaminated water at 48 and 72 h, respectively.This study has shown that contamination of water bodiesby carcasses of HS infected animals is a likely source of infection to heathy animals in the wild and that development of lesions severity is time dependant and significantly higher using contaminated water at 72 h than water contaminated for 24 and 48 h. This further indicates the danger of leaving decomposing carcasses in water bodies for prolonged periods.

American Journal of Animal and Veterinary Sciences
Volume 9 No. 2, 2014, 71-76

DOI: https://doi.org/10.3844/ajavsp.2014.71.76

Submitted On: 3 March 2014 Published On: 28 March 2014

How to Cite: Khaleel, M. M., Abdullah, F. F. J., Adamu, L., Abba, Y., Haron, A. W., Saad, M. Z. & Omar, A. R. (2014). HISTOPATHOLOGICAL CHANGES IN MICE INFECTED WITH RIVER WATER CONTAMINATED BY PASTEURELLA MULTOCIDA TYPE B:2. American Journal of Animal and Veterinary Sciences, 9(2), 71-76. https://doi.org/10.3844/ajavsp.2014.71.76

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Keywords

  • Pasteurella Multocida type B:2; Mice
  • Contaminated River Water
  • Histopathology