Research Article Open Access

The Ovario-Protective Effect of Erythropoietin against Oxidative Damage Associated with Reperfusion Following Ovarian Torsion in Rat

Sayyah-Melli Manizheh1, Kazemi-Shishvan Maryamalsadat1, Solaimani-Rad Jafar1, Rashidi Mohammad-Reza1, Roshangar Leila1, Rashtchizadeh Nadereh1, Ouladesahebmadarek Elaheh1, Ghojazadeh Morteza1 and Mashrabi Omid1
  • 1 Tabriz University of Medical Sciences, Iran

Abstract

Problem statement: To show the effect of recombinant Erythropoietin (rhEPO) on the ovarian viability and histology in the twisted ischemic ovaries in rats, followed by detorsion. Approach: An experimental study was conducted in primate clinic of the School of Medicine, Tabriz University of Medical Sciences from Dec. 2008 to Apr. 2009. Forty, 4 month old Wistar rats are cased in the present study divided into 4 groups. Ovarian ischemia was performed by torsion which was kept stable by using a vascular microclip for 4 h. In group 1, the ovary were surgically removed, fixed and analyzed histochemically. In group 2, the same procedure was repeated after 3 h reperfusion. In the next two groups, the same was performed rhEPO was administrated 400 u kg−1 1 h after torsion of the ovaries. Results: Thirty-two out of 40 rats were followed. There was a significant difference between groups in the levels of Malondialdehyde (MDA), Total Antioxidants (TA), Superoxidase (SOD), Nitric Oxide (NO) glutathione ( p<0.001). Addition of rhEPO maintained the normal histologic appearance in interventional groups compared with controls. Conclusion: Administration of rhEPO was effective in reducing the ischemic effect and free radicals damages of ovarian torsion in rats.

American Journal of Animal and Veterinary Sciences
Volume 6 No. 1, 2011, 18-24

DOI: https://doi.org/10.3844/ajavsp.2011.18.24

Submitted On: 13 October 2010 Published On: 26 April 2011

How to Cite: Manizheh, S., Maryamalsadat, K., Jafar, S., Mohammad-Reza, R., Leila, R., Nadereh, R., Elaheh, O., Morteza, G. & Omid, M. (2011). The Ovario-Protective Effect of Erythropoietin against Oxidative Damage Associated with Reperfusion Following Ovarian Torsion in Rat. American Journal of Animal and Veterinary Sciences, 6(1), 18-24. https://doi.org/10.3844/ajavsp.2011.18.24

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Keywords

  • Ovarian torsion
  • Analysis Of Variance (ANOVA)
  • Malondialdehyde (MDA)
  • oxidative
  • cytokine
  • Superoxidase (SOD)
  • ischemic effect