Research Article Open Access

Characterization of Escherichia coli Isolates from Commercial Layer Hens with Salpingitis

Mahmood Salehi1 and Reza Ghanbarpour1
  • 1 Shahid Bahonar University, Iran

Abstract

Problem statement: Escherichia coli isolates are the most common bacterial agents of salpingitis in commercial layer hens. The objectives of this study were to determine the phylogenetic groups/subgroups and antibiotic resistance of E. coli isolates from salpingitis cases in commercial layer hens farms in southeast of Iran. Approach: One hundred twenty one E. coli isolates from oviducts of layer hens with salpingitis were examined to determine their O-serogroup, phylogenetic group/subgroup and antibiotic resistance pattern. Results: O-serogroup determination test showed that 47 (38.84%) isolates were typeable and belonged to eleven different O serogroups including: O1, O2, O6, O8, O15, O20, O25, O36, O78, O86 and O111 and 71 (58.67%) isolates were O-nontypeable. Three most prevalent serogroup were O78, O2 and O1 respectively. PCR assays showed that the isolates fall into four phylogenetic groups A (41.32%), D (33.88%), B2 (14.87%) and B1 (9.91%). Ninety nine (81.81%) isolates fell into six phylogenetic subgroups including: A0 (17.35%), A1 (23.96%), B22 (5.78%), B23 (9.09%), D1 (13.22%) and D2 (20.66%). All of the E. coli isolates from O78 serogroup belonged to A phylo-group whereas the O2 isolates mostly fell into D group. The maximum antibiotic resistance was against tetracycline (100%) and minimum resistance was against linco-spectin (37.19%). Twenty four isolates (19.83%) were resistant to all of the examined antibiotics. Twenty three different patterns of multiple drug resistance were observed, out of which N-Te-Sxt-Fm- Nfx-C and N-Te-Sxt-Gm-Fm-Nfx-C-Ls was the two most frequent patterns respectively. The resistant isolates were found in all of the phylogenetic groups and or sub groups. Conclusion: There are similarities between salpingitis derived E. coli strains and other avian pathogenic E. coli isolates in phylogroups, O-serogroups and antibio-resistance patterns.

American Journal of Animal and Veterinary Sciences
Volume 5 No. 3, 2010, 208-214

DOI: https://doi.org/10.3844/ajavsp.2010.208.214

Submitted On: 5 September 2010 Published On: 30 September 2010

How to Cite: Salehi, M. & Ghanbarpour, R. (2010). Characterization of Escherichia coli Isolates from Commercial Layer Hens with Salpingitis. American Journal of Animal and Veterinary Sciences, 5(3), 208-214. https://doi.org/10.3844/ajavsp.2010.208.214

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Keywords

  • Escherichia coli
  • antibiotic
  • phylotyping
  • salpingitis