Research Article Open Access

Influence of the Prostatic Fluid from the First and Third Fractions of the Ejaculates on the Cryosurvival of Poodle Dog Sperm

Hideaki Yamashiro1, Kaori Narita2, Satoshi Sugimura1, Atsushi Sugawara1, Yumi Hoshino1, Masahiro Sakurai1, Masaki Yokoo1, Tsutomu Konno2, Muneyoshi Yoshida2 and Eimei Sato1
  • 1 Tohoku University, Japan
  • 2 Japan Animal Specialty College, Japan

Abstract

Problem statement: Two experiments were conducted to determine the effect of the prostatic fluid from the first and third fractions of the ejaculates on the freezability of poodle dog sperm. Approach: The ejaculates from 2 trained poodle dogs were collected. Effect of prostatic fluid from the first and third fractions of ejaculates on cryosurvival of dog sperm was examined. SDS-PAGE analyses were performed to verify whether there were any modifications in the protein patterns of the first and third fractions. Results: When second fractions of Dog A sperm were combined with the prostatic fluid from the first and third fractions of the ejaculates, sperm motility index was decreased compared to second fractions were collected alone. The post-thaw motility of Dog A sperm was significantly reduced when prostatic fluid from the first and third fractions of the ejaculates was added. The motility of frozen-thawed Dog B sperm did not appear to be altered when it was diluted with either of the prostatic fluids. The major bands present in both prostatic fluids corresponded to proteins with molecular masses of 30 and 75 kDa. The amount of proteins in the prostatic fluid from the third fraction was twice-three times as much protein present compared to the first fraction. Conclusion: Collection of the sperm-rich fraction along with either the prostatic fluid from the 1st and 3rd fractions of the ejaculates during sperm processing has varying detrimental effects on the motility characteristics of frozen-thawed sperm.

American Journal of Animal and Veterinary Sciences
Volume 4 No. 1, 2009, 14-20

DOI: https://doi.org/10.3844/ajavsp.2009.14.20

Submitted On: 7 March 2009 Published On: 31 March 2009

How to Cite: Yamashiro, H., Narita, K., Sugimura, S., Sugawara, A., Hoshino, Y., Sakurai, M., Yokoo, M., Konno, T., Yoshida, M. & Sato, E. (2009). Influence of the Prostatic Fluid from the First and Third Fractions of the Ejaculates on the Cryosurvival of Poodle Dog Sperm . American Journal of Animal and Veterinary Sciences, 4(1), 14-20. https://doi.org/10.3844/ajavsp.2009.14.20

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Keywords

  • Freezability
  • poodle dog sperm
  • prostatic fluid
  • raffinose
  • 30 kDa protein