Research Article Open Access

Noninvasive Measurement of Fecal Progesterone Concentration in Toy Poodles by Time Resolved Fluoroimmunoassay (TR-FIA)

Satoshi Sugimura1, Kaori Narita2, Hideaki Yamashiro1, Atsushi Sugawara1, Katsuhiko Nishimori1, Tsutomu Konno2, Muneyoshi Yoshida2 and Eimei Sato1
  • 1 Tohoku University, Japan
  • 2 Japan Animal Specialty College, Japan

Abstract

Progesterone is an important reproductive hormone and measurement of its level by repeated blood samplings is beneficial to monitoring of estrus cycle. However, since toy poodles have a small body size and thin-walled blood vessels, repeated blood samplings cause stress and affect their preparation for mating or artificial insemination (AI). Therefore, a noninvasive method for monitoring progesterone concentration should be developed. Here, we show that time-resolved fluoroimmunoassay (TF-RIA) is a useful noninvasive method for determining the progesterone concentration in serum and fecal samples obtained from toy poodles. Present results demonstrate that progesterone concentrations in the fecal correlated with the serum collected in same time and the sequential changes in progesterone concentrations in the feces are paralleled in the serum. Therefore, this technique may be suitable for monitoring the estrus cycle in toy poodles.

American Journal of Animal and Veterinary Sciences
Volume 3 No. 1, 2008, 43-46

DOI: https://doi.org/10.3844/ajavsp.2008.43.46

Submitted On: 28 February 2008 Published On: 31 March 2008

How to Cite: Sugimura, S., Narita, K., Yamashiro, H., Sugawara, A., Nishimori, K., Konno, T., Yoshida, M. & Sato, E. (2008). Noninvasive Measurement of Fecal Progesterone Concentration in Toy Poodles by Time Resolved Fluoroimmunoassay (TR-FIA). American Journal of Animal and Veterinary Sciences, 3(1), 43-46. https://doi.org/10.3844/ajavsp.2008.43.46

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Keywords

  • Fecal
  • Progesterone concentration
  • Time-resolved fluoroimmunoassay
  • Toy poodle