Research Article Open Access

Cellular and Some Biochemical Changes in Blood and Peritoneal Fluid Constituents in Awassi Lambs Following Elective Castration

Mohammad B.F. AL-Zghoul1, Raida K. AL-Rukibat1, Abdelsalam Q. Talafha1, Omar Ababneh1 and Zuhair A. Bani Ismail1
  • 1 Jordan University of Science and Technology, Jordan

Abstract

No reports could be cited in the recent literature concerning the effects of surgical castration on blood and peritoneal fluid constituents in lambs. The purpose of this study was to evaluate the effects of surgical castration on cellular and some biochemical parameters of blood and peritoneal fluid in Awassi lambs. Eight, clinically healthy, 5-month-old, Awassi lambs were surgically castrated. Peritoneal fluid and blood samples were collected prior to castration (day 0) and on days 1, 3 and 7 post castration. Total nucleated cell count (TNCC) was determined using an electric cell counter. Total protein (TP), albumin, fibrinogen, creatinine, urea, glucose, alkaline phosphatase (ALP), aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) concentrations were analyzed using commercially available kits. Following castration, the number of peripheral fluid white blood cells (WBC) and the percentage of neutrophils significantly increased (p<0.05) while the percentage of lymphocytes significantly decreased (p<0.05). The blood fibrinogen, urea, creatinine, glucose, ALP, AST and LDH concentrations significantly increased (p<0.05). However, TP concentrations did not change. In the peritoneal fluid analysis, the total WBC count and the percentage of neutrophils significantly increased (p<0.05) while the percentage of monocytes significantly decreased (p<0.05) following the operation. There was no significant change (p>0.05) in the percentage of lymphocytes in the peritoneal fluid. The concentration of peritoneal fluid TP, urea, creatinine, glucose, ALP and AST significantly increased (p<0.05) while LDH concentration did not change. Results of this study show that changes in the blood and peritoneal fluid cellular and biochemical components are likely to follow elective surgical castration in lambs. These changes must be taken in consideration when peritoneal fluid samples from lambs are evaluated for the diagnosis of abdominal diseases.

American Journal of Animal and Veterinary Sciences
Volume 3 No. 1, 2008, 23-27

DOI: https://doi.org/10.3844/ajavsp.2008.23.27

Submitted On: 13 March 2008 Published On: 31 March 2008

How to Cite: AL-Zghoul, M. B., AL-Rukibat, R. K., Talafha, A. Q., Ababneh, O. & Bani Ismail, Z. A. (2008). Cellular and Some Biochemical Changes in Blood and Peritoneal Fluid Constituents in Awassi Lambs Following Elective Castration . American Journal of Animal and Veterinary Sciences, 3(1), 23-27. https://doi.org/10.3844/ajavsp.2008.23.27

  • 3,215 Views
  • 3,635 Downloads
  • 2 Citations

Download

Keywords

  • Awassi lambs
  • castration
  • inflammation
  • peritoneal fluid