American Journal of Biochemistry and Biotechnology

Sex Identification and the Sex-Related Genes of Nipponia Nippon

Feilong Sun, Xuanfu Huang and Shuiyun Yang

DOI : 10.3844/ajbbsp.2017.81.84

American Journal of Biochemistry and Biotechnology

Volume 13, Issue 2

Pages 81-84


Sex identification in birds is necessary for studying their ecology, behavior and population dynamics and structure. This study was carried out to determine the sex of Nipponia nippon by a Polymerase Chain Reaction (PCR) method. The sexing universal primers of non-ratite birds were employed to amplify the sexing related genes and identify the sex of each individual. A 657 bp CHD gene fragments on Z chromosome in both sexes and a 463 bp female specific CHD fragments on W chromosome were cloned and sequenced. The sequences of these two sexing related genes fragments were similar with CHD-W of Phalacrocorax capillatuss and CHD-Z of Platalea minor individually. This study is valuable for managing and conserving endangered birds.


© 2017 Feilong Sun, Xuanfu Huang and Shuiyun Yang. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.