TY  - JOUR
AU  - Sun, Feilong 
AU  - Huang, Xuanfu 
AU  - Yang, Shuiyun 
PY  - 2017
TI  - Sex Identification and the Sex-Related Genes of Nipponia Nippon
JF  - American Journal of Biochemistry and Biotechnology
VL  - 13
IS  - 2
DO  - 10.3844/ajbbsp.2017.81.84
UR  - https://thescipub.com/abstract/ajbbsp.2017.81.84
AB  - Sex identification in birds is necessary for studying their ecology, behavior and population dynamics and structure. This study was carried out to determine the sex of Nipponia nippon by a Polymerase Chain Reaction (PCR) method. The sexing universal primers of non-ratite birds were employed to amplify the sexing related genes and identify the sex of each individual. A 657 bp CHD gene fragments on Z chromosome in both sexes and a 463 bp female specific CHD fragments on W chromosome were cloned and sequenced. The sequences of these two sexing related genes fragments were similar with CHD-W of Phalacrocorax capillatuss and CHD-Z of Platalea minor individually. This study is valuable for managing and conserving endangered birds.