@article {10.3844/ajbbsp.2017.81.84, article_type = {journal}, title = {Sex Identification and the Sex-Related Genes of Nipponia Nippon}, author = {Sun, Feilong and Huang, Xuanfu and Yang, Shuiyun}, volume = {13}, number = {2}, year = {2017}, month = {May}, pages = {81-84}, doi = {10.3844/ajbbsp.2017.81.84}, url = {https://thescipub.com/abstract/ajbbsp.2017.81.84}, abstract = {Sex identification in birds is necessary for studying their ecology, behavior and population dynamics and structure. This study was carried out to determine the sex of Nipponia nippon by a Polymerase Chain Reaction (PCR) method. The sexing universal primers of non-ratite birds were employed to amplify the sexing related genes and identify the sex of each individual. A 657 bp CHD gene fragments on Z chromosome in both sexes and a 463 bp female specific CHD fragments on W chromosome were cloned and sequenced. The sequences of these two sexing related genes fragments were similar with CHD-W of Phalacrocorax capillatuss and CHD-Z of Platalea minor individually. This study is valuable for managing and conserving endangered birds.}, journal = {American Journal of Biochemistry and Biotechnology}, publisher = {Science Publications} }