Effect of Cinnamomum cassia Stem-Bark Extracts on the Synthesis and Secretion of Insulin in RIN-m5F Cells
- 1 Sefako Makgatho Health Sciences University, South Africa
- 2 Tshwane University of Technology, South Africa
Cinnamomum cassia (C. cassia) crude stem-bark extracts possess some hypoglycemic properties through inhibiting the uptake of glucose by the intestines and/or enhancing insulin sensitivity. To further investigate the mechanism through which the hypoglycemic effect is exerted, this research explored the influence of the extracts of the crude stem-bark of C. cassia, with various polarities, on particular aspects of glucose consumption as well as glucose-stimulated insulin synthesis and secretion by RIN-m5F cells. All C. cassia extracts did not enhance glucose consumption when compared to the untreated cells (p˂0.001). However, lower concentrations of the extracts significantly increased mRNA levels of Glucose Transporter 2 (GLUT2) and glucokinase genes. Furthermore, all C. cassia extracts significantly stimulated/increased total insulin synthesis (intracellular and secreted) when compared with the control cells, though the effect was not dose-dependent except for water extracts at higher concentration (p<0.001). The results of this investigation revealed that generally, the extracts upregulated the expression of Musculoaponeurotic fibrosarcoma homolog A (MafA), the transcription factor involved in insulin gene expression, while that of the Pancreatic Duodenal Homeobox-1 (PDX-1) gene was mostly suppressed. In concluding, the results of this study proposes that C. cassia stem-bark extracts may exert hypoglycemic effects through stimulating insulin synthesis and secretion in the RIN-m5F pancreatic beta cells.
Copyright: © 2022 Ananias Hodi Kgopa, Sebolaishi Doris Makhubela, Motetelo Alfred Mogale and Leshweni Jerry Shai. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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- Pancreatic Beta Cells
- Cinnamomum cassia
- Glucose Uptake
- Synthesis and Secretion of Insulin
- MafA and PDX-1