Research Article Open Access

Advances in Menaquinone-7 Production by Bacillus subtilis Natto: Fed-Batch Glycerol Addition

Aydin Berenjian1, Raja Mahanama1, Andrea Talbot2, Hubert Regtop2, John Kavanagh1 and Fariba Dehghani1
  • 1 University of Sydney, Australia
  • 2 , Australia


Menaquinone-7 was produced by fermentation mainly using Bacillus subtilis species. Due to the low concentration of menaquinone-7 in fermentation processes and the associated expense of recovering the menaquinone-7, it was desirable to improve the fermentation concentration. The aim of this study was to determine the effect of fed-batch glycerol addition in the production of menaquinone-7 during the fermentation of Bacillus subtilis natto in both small (25-mL) and bench scale (3-L) fermenters. Liquid phase fermentation was carried out aerobically at 40°C for a period of 6 days. The pH, bacterial growth, glycerol and menaquinone-7 concentrations were measured every day. Maximum menaquinone-7 was produced when 2% (w/v) glycerol was added to the fermentation media in the second day (48 h) of fermentation. The results were demonstrated 40% increase of menaquinone-7 concentration compared to batch culture. This study provides valuable data for further optimization and scale-up the menaquinone-7 fermentation process.

American Journal of Biochemistry and Biotechnology
Volume 8 No. 2, 2012, 105-110


Submitted On: 9 May 2012 Published On: 22 June 2012

How to Cite: Berenjian, A., Mahanama, R., Talbot, A., Regtop, H., Kavanagh, J. & Dehghani, F. (2012). Advances in Menaquinone-7 Production by Bacillus subtilis Natto: Fed-Batch Glycerol Addition. American Journal of Biochemistry and Biotechnology, 8(2), 105-110.

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  • Menaquinone-7
  • glycerol
  • fed-batch
  • fermentation
  • Bacillus subtilis natto