@article {10.3844/ajbbsp.2012.105.110,
article_type = {journal},
title = {Advances in Menaquinone-7 Production by Bacillus subtilis Natto: Fed-Batch Glycerol Addition},
author = {Berenjian, Aydin and Mahanama, Raja and Talbot, Andrea and Regtop, Hubert and Kavanagh, John and Dehghani, Fariba},
volume = {8},
number = {2},
year = {2012},
month = {Jun},
pages = {105-110},
doi = {10.3844/ajbbsp.2012.105.110},
url = {https://thescipub.com/abstract/ajbbsp.2012.105.110},
abstract = {Menaquinone-7 was produced by fermentation mainly using Bacillus subtilis species. Due to the low concentration of menaquinone-7 in fermentation processes and the associated expense of recovering the menaquinone-7, it was desirable to improve the fermentation concentration. The aim of this study was to determine the effect of fed-batch glycerol addition in the production of menaquinone-7 during the fermentation of Bacillus subtilis natto in both small (25-mL) and bench scale (3-L) fermenters. Liquid phase fermentation was carried out aerobically at 40°C for a period of 6 days. The pH, bacterial growth, glycerol and menaquinone-7 concentrations were measured every day. Maximum menaquinone-7 was produced when 2% (w/v) glycerol was added to the fermentation media in the second day (48 h) of fermentation. The results were demonstrated 40% increase of menaquinone-7 concentration compared to batch culture. This study provides valuable data for further optimization and scale-up the menaquinone-7 fermentation process.},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}