Research Article Open Access

In Vitro Interaction of 5-Hydroxytrptamine with Cytosolic Molybdenum Hydroxylases as a Potential Inhibitor for Initial Rates Activities

Abdullah M. Al-Mohizea1, Abd El-Galil E. Amr1 and Mohamed A. Al-Omar1
  • 1 King Saud University, Saudi Arabia

Abstract

Problem statement: The role of 5-HT has been investigated in many behavioral activities. Thus, studies using raphe lesion showed that 5-HT is involved in sleep, general activity levels, habituation, aggression, pain sensitivity and morphine analgesia, avoidance behavior, self-stimulation and water consumption. Approach: The metabolic interaction between serotonin (5- hydroxytrptamine) and indole-3-aldehyde and xanthine via aldehyde oxidase (EC 1.2.3.1) and xanthine oxidase (EC 1.1.3.22), respectively, were studied in liver tissue homogenate of Dunkin-Hartley guinea pigs by following the decrease in substrate concentration using spectrophotometer. Homogenates of liver were incubated with indole-3-aldehyde in the presence and absence of serotonin or (chlorpromazine and allopurinol a potent and selective inhibitors for aldehyde oxidase and xanthine oxidase, respectively). Oxidation of indole-3-aldehyde to indole-3-acetic acid was reduced up to 63.2% in the presence of serotonin (100 µM), while oxidation of xanthine to uric acid was reduced up to 51.6% under the same conditions. Results: In comparison, incubation of the substrates with their specific inhibitors (100 µM of chlorpromazine and 100 µM allopurinol) give almost complete inhibition. These results demonstrate that in the guinea pig liver a metabolic interaction between serotonin and indole-3-aldehyde or xanthine via molybdenum hydroxylases system may take place in liver, which is the main tissue for xenobiotics detoxification. Conclusion: The overall conclusion from this research is that serotonin could be a protector for neurons and other tissue from the insult of oxidation of aldehydes and xanthines by molybdenum hydroxylases.

American Journal of Biochemistry and Biotechnology
Volume 6 No. 3, 2010, 181-186

DOI: https://doi.org/10.3844/ajbbsp.2010.181.186

Submitted On: 14 August 2010 Published On: 30 September 2010

How to Cite: Al-Mohizea, A. M., Amr, A. E. E. & Al-Omar, M. A. (2010). In Vitro Interaction of 5-Hydroxytrptamine with Cytosolic Molybdenum Hydroxylases as a Potential Inhibitor for Initial Rates Activities. American Journal of Biochemistry and Biotechnology, 6(3), 181-186. https://doi.org/10.3844/ajbbsp.2010.181.186

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Keywords

  • Indole-3-aldehyde
  • 5-hydroxytrptamine
  • β-estradiol
  • aldehyde oxidase
  • xanthine oxidase