@article {10.3844/ajbbsp.2010.181.186,
article_type = {journal},
title = {In Vitro Interaction of 5-Hydroxytrptamine with Cytosolic Molybdenum Hydroxylases as a Potential Inhibitor for Initial Rates Activities},
author = {Al-Mohizea, Abdullah M. and Amr, Abd El-Galil E. and Al-Omar, Mohamed A.},
volume = {6},
number = {3},
year = {2010},
month = {Sep},
pages = {181-186},
doi = {10.3844/ajbbsp.2010.181.186},
url = {https://thescipub.com/abstract/ajbbsp.2010.181.186},
abstract = {Problem statement: The role of 5-HT has been investigated in many behavioral activities.
Thus, studies using raphe lesion showed that 5-HT is involved in sleep, general activity levels,
habituation, aggression, pain sensitivity and morphine analgesia, avoidance behavior, self-stimulation
and water consumption. Approach: The metabolic interaction between serotonin (5-
hydroxytrptamine) and indole-3-aldehyde and xanthine via aldehyde oxidase (EC 1.2.3.1) and xanthine
oxidase (EC 1.1.3.22), respectively, were studied in liver tissue homogenate of Dunkin-Hartley guinea
pigs by following the decrease in substrate concentration using spectrophotometer. Homogenates of
liver were incubated with indole-3-aldehyde in the presence and absence of serotonin or
(chlorpromazine and allopurinol a potent and selective inhibitors for aldehyde oxidase and xanthine
oxidase, respectively). Oxidation of indole-3-aldehyde to indole-3-acetic acid was reduced up to 63.2%
in the presence of serotonin (100 µM), while oxidation of xanthine to uric acid was reduced up to 51.6% under the same conditions. Results: In comparison, incubation of the substrates with their specific inhibitors (100 µM of chlorpromazine and 100 µM allopurinol) give almost complete inhibition. These results demonstrate that in the guinea pig liver a metabolic interaction between serotonin and indole-3-aldehyde or xanthine via molybdenum hydroxylases system may take place in liver, which is the main tissue for xenobiotics detoxification. Conclusion: The overall conclusion from this research is that serotonin could be a protector for neurons and other tissue from the insult of oxidation of aldehydes and xanthines by molybdenum hydroxylases.},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}