Research Article Open Access

Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

Mohd Sahaid Kalil1, Hisham Salem Alshiyab1 and Wan Mohtar Wan Yusoff1
  • 1 University Kebangsaan Malaysia 43600 UKM Bangi Selangor, Malaysia

Abstract

Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the nitrogen source and its concentration. Approach: Using different nitrogen source with different concentration on bacterial productivity of hydrogen showed to affect on both bacterial productivity of hydrogen and biomass concentration. Results: Yeast extract as nitrogen source at concentration of 13 g L-1 was the best organic nitrogen source and resulted in hydrogen yield YP/S of 308 mL g-1 glucose utilized with biomass concentration of 1.1 g L-1, hydrogen yield per biomass YP/X of 280 mL g-1 L-1, biomass per substrate utilized YX/S of 0.22 and produced hydrogen in gram per gram of glucose utilized YH2/S of 0.0275. C/N of 70 enhanced the YP/S from 308-350 mL g-1 glucose utilized with biomass concentration of 1.22 gL-1, YP/X of 287 mL g-1 L-1, YX/S of 0.244 and YH2/S of 0.03125. Conclusion: Nitrogen source with proper C:N ratio enhanced the hydrogen production.

American Journal of Biochemistry and Biotechnology
Volume 4 No. 4, 2008, 393-401

DOI: https://doi.org/10.3844/ajbbsp.2008.393.401

Submitted On: 28 November 2008 Published On: 31 December 2009

How to Cite: Kalil, M. S., Alshiyab, H. S. & Wan Yusoff, W. M. (2008). Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum. American Journal of Biochemistry and Biotechnology, 4(4), 393-401. https://doi.org/10.3844/ajbbsp.2008.393.401

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Keywords

  • Hydrogen production
  • clostridium
  • anaerobic fermentation
  • yeast extract