@article {10.3844/ajbbsp.2008.393.401, article_type = {journal}, title = {Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum}, author = {Kalil, Mohd Sahaid and Alshiyab, Hisham Salem and Wan Yusoff, Wan Mohtar}, volume = {4}, number = {4}, year = {2009}, month = {Dec}, pages = {393-401}, doi = {10.3844/ajbbsp.2008.393.401}, url = {https://thescipub.com/abstract/ajbbsp.2008.393.401}, abstract = {Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the nitrogen source and its concentration. Approach: Using different nitrogen source with different concentration on bacterial productivity of hydrogen showed to affect on both bacterial productivity of hydrogen and biomass concentration. Results: Yeast extract as nitrogen source at concentration of 13 g L-1 was the best organic nitrogen source and resulted in hydrogen yield YP/S of 308 mL g-1 glucose utilized with biomass concentration of 1.1 g L-1, hydrogen yield per biomass YP/X of 280 mL g-1 L-1, biomass per substrate utilized YX/S of 0.22 and produced hydrogen in gram per gram of glucose utilized YH2/S of 0.0275. C/N of 70 enhanced the YP/S from 308-350 mL g-1 glucose utilized with biomass concentration of 1.22 gL-1, YP/X of 287 mL g-1 L-1, YX/S of 0.244 and YH2/S of 0.03125. Conclusion: Nitrogen source with proper C:N ratio enhanced the hydrogen production.}, journal = {American Journal of Biochemistry and Biotechnology}, publisher = {Science Publications} }