Research Article Open Access

Platelet Derived Growth Factor-A mRNA Levels in Diabetic and Nondiabetic Subjects at Risk of Coronary Heart Disease

Salman A.H. Alrokayan1
  • 1 King Saud University, Saudi Arabia

Abstract

Coronary heart disease (CHD) is the number one cause of death in developed countries. We investigated the quantity of platelet derived growth factor-A mRNA (PDGF-A mRNA) among four different groups of diabetic and nondiabetic Saudi subjects (n = 40) at risk of CHD. Total RNA was derived from peripheral blood mononuclear cells (PBMN) and PDGF-A mRNA reverse transcribed into cDNA in the presence of internal standard (cRNA) and amplified by competitive reverse transcriptase polymerase chain reaction (cRT-PCR). The gel was silver stained after polyacrylamide gel electrophoresis (PAGE) and PDGF-A mRNA quantitated by scanning densitometry. We demonstrate a significantly high expression of PDGF-A mRNA in hyperlipidaemic and diabetic subjects (41 ± 4 × 104 copies/µg of RNA) compared to normal healthy individuals (29.3 ± 5 × 104 copies/µg of RNA). These findings suggest an important role of PDGF-A mRNA in progression of CHD among diabetics.

American Journal of Biochemistry and Biotechnology
Volume 3 No. 3, 2007, 167-170

DOI: https://doi.org/10.3844/ajbbsp.2007.167.170

Submitted On: 8 May 2007 Published On: 30 September 2007

How to Cite: Alrokayan, S. A. (2007). Platelet Derived Growth Factor-A mRNA Levels in Diabetic and Nondiabetic Subjects at Risk of Coronary Heart Disease. American Journal of Biochemistry and Biotechnology, 3(3), 167-170. https://doi.org/10.3844/ajbbsp.2007.167.170

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Keywords

  • PDGF-A
  • mRNA quantitation
  • cRT-PCR
  • CHD
  • Diabetes mellitus
  • Atherosclerosis