TY  - JOUR
AU  - Alrokayan, Salman A.H. 
PY  - 2007
TI  - Platelet Derived Growth Factor-A mRNA Levels in Diabetic and Nondiabetic Subjects at Risk of Coronary Heart Disease
JF  - American Journal of Biochemistry and Biotechnology
VL  - 3
IS  - 3
DO  - 10.3844/ajbbsp.2007.167.170
UR  - https://thescipub.com/abstract/ajbbsp.2007.167.170
AB  - Coronary heart disease (CHD) is the number one cause of death in developed countries. We investigated the quantity of platelet derived growth factor-A mRNA (PDGF-A mRNA) among four different groups of diabetic and nondiabetic Saudi subjects (n = 40) at risk of CHD. Total RNA was derived from peripheral blood mononuclear cells (PBMN) and PDGF-A mRNA reverse transcribed into cDNA in the presence of internal standard (cRNA) and amplified by competitive reverse transcriptase polymerase chain reaction (cRT-PCR). The gel was silver stained after polyacrylamide gel electrophoresis (PAGE) and PDGF-A mRNA quantitated by scanning densitometry. We demonstrate a significantly high expression of PDGF-A mRNA in hyperlipidaemic and diabetic subjects (41 ± 4 × 104 copies/µg of RNA) compared to normal healthy individuals (29.3 ± 5 × 104 copies/µg of RNA). These findings suggest an important role of PDGF-A mRNA in progression of CHD among diabetics.