OnLine Journal of Biological Sciences

Non-Target Inhibition of Antioxidant Enzymes in Honey Bees (A. mellifera and A. florea) Upon Pesticide Exposure

Amal Ahmed Berjawi, Syed Salman Ashraf and Mohammad Ali Al-Deeb

DOI : 10.3844/ojbsci.2020.57.65

OnLine Journal of Biological Sciences

Volume 20, Issue 2

Pages 57-65


Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.


© 2020 Amal Ahmed Berjawi, Syed Salman Ashraf and Mohammad Ali Al-Deeb. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.