TY  - JOUR
AU  - Berjawi, Amal Ahmed 
AU  - Ashraf, Syed Salman 
AU  - Al-Deeb, Mohammad Ali 
PY  - 2020
TI  - Non-Target Inhibition of Antioxidant Enzymes in Honey Bees (A. mellifera and A. florea) Upon Pesticide Exposure
JF  - OnLine Journal of Biological Sciences
VL  - 20
IS  - 2
DO  - 10.3844/ojbsci.2020.57.65
UR  - https://thescipub.com/abstract/ojbsci.2020.57.65
AB  - Understanding how different insecticides influence bee health is very essential. The aim of this work was to evaluate enzyme kinetics and inhibition by insecticides in Apis mellifera and Apis florea. Tested enzymes showed variable affinity to substrates. In A. florea, glutathione S-transferase (GST) showed significantly higher affinity for its 1-Chloro-2,4-Dinitrobenzene (CDNB) substrate than in A. mellifera. Carbosulfan caused the highest inhibition to acetylcholinesterase (AChE) in both tested species in comparison with chlorpyrifos, dimethoate and pirimiphos-methyl. Our results show that exposure to insecticides is harmful to honey bees and results in negative effects on enzyme activity.