Research Article Open Access

CYTOTOXICITY AND MODE OF CELL DEATH INDUCED BY TRIPHENYLTIN (IV) COMPOUNDS IN VITRO

Normah Awang1, Zalila Abdul Aziz1, Nurul Farahana Kamaludin1 and Kok Meng Chan1
  • 1 Universiti Kebangsaan Malaysia, Malaysia

Abstract

A series of newly synthesized organotin (IV) with N-alkyl-N-phenyldithiocarbamate ligands namely triphenyltin (IV) ethylphenyldithiocarbamate (compound 1) and triphenyltin (IV) butylphenyldithiocarbamate (compound 2) were assessed for their cytotoxic effect against HT-29 human colon adenocarcinoma cells and human CCD-18Co normal colon cells. The cytotoxicity of these organotins in both cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazholium bromide (MTT) assay upon 24 h treatment. Both compounds demonstrated potent cytotoxicity towards HT-29 cells with the IC50 of 0.18 µM for compound 1 and 0.20 µM for compound 2. Interestingly, compound 1 exhibited lower cytotoxicity towards CCD-18Co with IC50 of 1.55 µM whereas no IC50 was detected for compound 2 up to 2 µM treatment. The mode of cell death was determined based on the externalization of phosphatidylserine using flow cytometry. Cells treated with compound 1 and compound 2 were mainly viable and the apoptotic cell death was around 10% which suggests that both compounds induced growth arrest. In conclusion, this study demonstrated that both compounds were selective towards human colorectal cells by giving a strong cytotoxicity to cancer cells and low toxicity towards normal cells. Both compounds were suggested to induce growth arrest in HT-29 cells.

OnLine Journal of Biological Sciences
Volume 14 No. 2, 2014, 84-93

DOI: https://doi.org/10.3844/ojbsci.2014.84.93

Submitted On: 25 February 2014 Published On: 15 March 2014

How to Cite: Awang, N., Aziz, Z. A., Kamaludin, N. F. & Chan, K. M. (2014). CYTOTOXICITY AND MODE OF CELL DEATH INDUCED BY TRIPHENYLTIN (IV) COMPOUNDS IN VITRO. OnLine Journal of Biological Sciences, 14(2), 84-93. https://doi.org/10.3844/ojbsci.2014.84.93

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Keywords

  • Cytotoxicity
  • Apoptosis
  • Organotin (IV)
  • Dithiocarbamate
  • HT-29
  • CCD-18Co