@article {10.3844/ojbsci.2014.84.93,
article_type = {journal},
title = {CYTOTOXICITY AND MODE OF CELL DEATH INDUCED BY TRIPHENYLTIN (IV) COMPOUNDS  IN VITRO},
author = {Awang, Normah and Aziz, Zalila Abdul and Kamaludin, Nurul Farahana and Chan, Kok Meng},
volume = {14},
number = {2},
year = {2014},
month = {Mar},
pages = {84-93},
doi = {10.3844/ojbsci.2014.84.93},
url = {https://thescipub.com/abstract/ojbsci.2014.84.93},
abstract = {A series of newly synthesized organotin (IV) with N-alkyl-N-phenyldithiocarbamate ligands namely triphenyltin (IV) ethylphenyldithiocarbamate (compound 1) and triphenyltin (IV) butylphenyldithiocarbamate (compound 2) were assessed for their cytotoxic effect against HT-29 human colon adenocarcinoma cells and human CCD-18Co normal colon cells.  The cytotoxicity of these organotins in both cells was assessed using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazholium bromide (MTT) assay upon 24 h treatment. Both compounds demonstrated potent cytotoxicity towards HT-29 cells with the IC50 of 0.18 µM for compound 1 and 0.20 µM for compound 2. Interestingly, compound 1 exhibited lower cytotoxicity towards CCD-18Co with IC50 of 1.55 µM whereas no IC50 was detected for compound 2 up to 2 µM treatment. The mode of cell death was determined based on the externalization of phosphatidylserine using flow cytometry. Cells treated with compound 1 and compound 2 were mainly viable and the apoptotic cell death was around 10% which suggests that both compounds induced growth arrest. In conclusion, this study demonstrated that both compounds were selective towards human colorectal cells by giving a strong cytotoxicity to cancer cells and low toxicity towards normal cells. Both compounds were suggested to induce growth arrest in HT-29 cells.},
journal = {OnLine Journal of Biological Sciences},
publisher = {Science Publications}
}