Apoptosis of human Breast Cancer Cells induced by Ethylacetate Extracts of Propolis
Syamsudin , Partomuan Simanjuntak, Ratna Djamil and Wan Lelly Heffen
DOI : 10.3844/ajbbsp.2010.84.88
American Journal of Biochemistry and Biotechnology
Volume 6, Issue 2
Problem statement: Propolis has been ethno medically claimed to possess a wide array of biological activities including anticancer activity. The purpose of this research was to verify the folklore claim. Approach: This study was performed in a human breast carcinoma cell, MCF-7. Extract of propolis from different solvent, ethylacetate and n-buthanol showed induced apoptotic cells was detected by flow cytometry. Results: The results demonstrated that ethylacetate extract of propolis can induce apoptosis in MCF-7 as large as 13.21% during the 24 h incubation. On the other hand, doxorubicin is able to induce apoptosis as large as 18.89% during the 24 h incubation. Conclusion: The extracts of propolis ethylacetate had cytotoxic activity and triggers apoptosis on MCF-7 cells.
© 2010 Syamsudin , Partomuan Simanjuntak, Ratna Djamil and Wan Lelly Heffen. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.