Research Article Open Access

Phytochemical Composition, Antibacterial, and Antioxidant Properties of Foeniculum vulgare Essential Oil

Saif M. Dmour1, Saqr Abushattal1, Sultan Ayesh Mohammed Saghir1, Sulaiman Mohammed Alnaimat1, Haitham Qaralleh2, Ahmad Mohammed Al-Jaafreh2 and Eid Musa Alsbou3
  • 1 Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Health Sciences, Al-Hussein Bin Talal University, Jordan
  • 2 Department of Medical Laboratory Sciences, Mutah University, Mutah, Jordan
  • 3 Department of Chemistry, Science College, Al-Hussein Bin Talal University, Ma'an, Jordan

Abstract

This study intended to inspect the phytochemical composition and bioactivity of Fennel (Foeniculum vulgare) Seed Essential Oil (FSEO) from Jordan. The EO was extracted via hydrodistillation from air-dried samples with 4.42% (v/w) yield. Twenty compounds, constituting around 98.5% of the EO were identified using Gas Chromatography-Mass Spectrometry (GC-MS). Estragole (47.15%) and Anethole (36.2%), appeared as predominant oxygenated monoterpenes compounds in the FSEO. Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) assays were conducted to assess the antimicrobial potential of FSEO against various strains with MIC90 values ranging from 2.13 mg/ml to 2.76 mg/ml. Notably, FSEO exhibited a significant activity against MRSE and S. aureus. Furthermore, FSEO demonstrated substantial phenolic content (39.59±0.062 mg GAE/g dry plant material), flavonoid content (20.95±0.146 mg CE/g dry extract), and tannin (7.15±0.1 mg QE/g dry extract). Antioxidant efficacy, evaluated using DPPH and ABTS assays revealed dose-dependent scavenging activity, with IC50 values of 126.05±1.01 µg/mL and 49.24±1.02 µg/mL, respectively. These findings highlight the potential of FSEO as an antimicrobial and antioxidant agent.

OnLine Journal of Biological Sciences
Volume 25 No. 3, 2025, 617-626

DOI: https://doi.org/10.3844/ojbsci.2025.617.626

Submitted On: 20 November 2024 Published On: 1 August 2025

How to Cite: Dmour, S. M., Abushattal, S., Saghir, S. A. M., Alnaimat, S. M., Qaralleh, H., Al-Jaafreh, A. M. & Alsbou, E. M. (2025). Phytochemical Composition, Antibacterial, and Antioxidant Properties of Foeniculum vulgare Essential Oil. OnLine Journal of Biological Sciences, 25(3), 617-626. https://doi.org/10.3844/ojbsci.2025.617.626

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Keywords

  • MRSA
  • DPPH
  • GC-MS
  • MIC
  • Foeniculum vulgare
  • Estragole