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Research Article Open Access

Wild Allium longicuspis Regel is a Feral Form of Allium sativum L. in Kazakhstan: A Comparative Molecular Genetic Analysis

Akerke Tokenova1,2, Gulnar Sitpayeva2, Nadezhda Gemejiyeva2, Saule Suleimenova1, Nikolai Friesen3, Nikolai Friesen 4 and Dariga Batayeva5
  • 1 Department of Agronomy, Kazakh National Agrarian University, Almaty, Kazakhstan
  • 2 Republican State-Owned Enterprise on the Right of Economic Management "Institute of Botany and Phytointroduction" of Forestry and Wildlife Committee of the Ministry of Ecology, Geology and Natural Resources of the Republic of Kazakhstan, Almaty, Kazakhstan
  • 3 Botanical Garden of the University of Osnabrück, Osnabrück, Germany
  • 4 Department of Pharmaceutical and Natural Sciences, I.M. Sechenov First Moscow State Medical University, Moscow, Russia
  • 5 Department of Biology, Kazakh National Women’s Teacher Training University, Almaty, Kazakhstan

Abstract

The genus Allium L comprises over 1000 species with important nutritional, medicinal and horticultural applications. The natural flora of Kazakhstan is rich in Allium species, including the wild long-pointed onion, A. longicuspis and cultivated A. sativum garlic varieties. It has been hypothesized that A. longicupis is ancestral to A. sativum. We investigated genetic polymorphism in samples of A. longicupis and the A. sativum cultivars “Niki” and “Merey” using start codon targeted polymorphism analysis. Nuclear ribosomal DNA fragments were purified, amplified and tested with 25 primers, finally selecting 12 primers that identified polymorphisms. DNA samples were sequenced, electrophoresed and cluster analysis performed by the construction of dendrograms and principal component analysis. The karyotypes of the different samples were also compared. The results showed that the samples of A. longicupis from natural populations did not differ significantly from those of the cultivated garlic varieties. The results indicate that A. longicupis can be considered a feral, rather than an ancestral, form of A. sativum. These findings have implications both for the breeding and cultivation of garlic varieties and for the taxonomy of the Allium genus. 

OnLine Journal of Biological Sciences
Volume 23 No. 1, 2023, 33-43

DOI: https://doi.org/10.3844/ojbsci.2023.33.43

Submitted On: 25 July 2022 Published On: 26 December 2022

How to Cite: Tokenova, A., Sitpayeva, G., Gemejiyeva, N., Suleimenova, S., Friesen, N., Friesen , N. & Batayeva, D. (2023). Wild Allium longicuspis Regel is a Feral Form of Allium sativum L. in Kazakhstan: A Comparative Molecular Genetic Analysis. OnLine Journal of Biological Sciences, 23(1), 33-43. https://doi.org/10.3844/ojbsci.2023.33.43

Open AccessCopyright: © 2023 Akerke Tokenova, Gulnar Sitpayeva, Nadezhda Gemejiyeva, Saule Suleimenova, Nikolai Friesen, Nikolai Friesen and Dariga Batayeva. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Garlic
  • Long-Pointed Onion
  • SCoT Method
  • Karyotype
  • Population