Enhancement of in vitro Rooting through Growth Media, Gelling Agents and Activated Charcoal in Lycium chinense

Abstract

Lycium chinense, a traditional Chinese herbal medicine is well known for its medicinal value and composition for centuries in Asia. Plant tissue culture technique has proven as a quick and sustainable ways to regenerate various plants species those are valuable in terms of medicinal as well as ornamental. Here, we investigated the in vitro root regeneration and root growth of L. chinense in response of different media, gelling agents and activated charcoal. The growth media Schenk and Hildebrandt medium (SH medium) performed the best for being the highest number of roots in each (3.50) and also for the longest root length (33.80 mm) exhibiting 20% and 18% higher roots/explant and root growth, respectively than that of the lowest roots/explant and root growth containg medium of MS. The highest number of roots (3.90) in each explant was observed when phytagar at 6 g L^-1 has been used in the culture media producing a 26% higher number of roots in each explant. Phytagar at the lowest concentration (5 g L^-1) used in this study produced the longest root length (35.10 mm) exhibiting 20% higher root length than that of the highest concentration (9 g L^-1) of phytagar and after that with further increase the concentration of Phytagar the growth of the root length has been decreased. Here concentration of Gelrite at 3 g L^-1 responded positively for getting the highest number of roots (4.00) in each explants and the longest root length (35.2 mm). Activated charcoal at 1 g L^-1 produced the highest roots (4.10) in each explant and also enhanced to produce the longest (45.40 mm) root length these findings could contributed to enhance rooting ability in any crops especially some medical and ornamental cops and even could provide useful information for future industrial-scale root production of L. chinense.