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Frequency: Quarterly
ISSN: 1608-4217 (Print)
ISSN: 2410-8561 (Online)
Research Article Open Access

Effect of Serial Passaging on the Morphology and Biological Characteristics of Human Adipose Tissue-derived Stem Cells

Lenka Oravcova1, Martin Bohac2, Lubica Krajciova3, Stefan Polak1, Daniel Bohmer4 and Lubos Danisovic4
  • 1 Institute of Histology and Embryology, Faculty of Medicine, Comenius University, Bratislava, Slovakia
  • 2 Department of Plastic Surgery, Faculty of Medicine, Comenius University, Bratislava, Slovakia
  • 3 Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Bratislava, Slovakia
  • 4 Institute of Medical Biology, Genetics and Clinical Genetics, Faculty of Medicine, Comenius University, Bratislava, Slovakia, Slovakia

Abstract

Somatic stem cells posses an exclusive property of self regeneration and plasticity which makes them promising candidates for use in tissue engineering and regenerative medicine. In the case of stem cell therapy it is crucial to obtain them in sufficient amount. That's why they have to be cultured under in vitro conditions. Long term culture provides adequate number of cells, but on the other hand it is associated with increased risk of spontaneous cell transformation. In this study we analyzed the effect of serial passaging (up to 10th passage) on the morphological and selected biological characteristics of human stem cells isolated from adipose tissue in respect to potential malignant transformation. Obtained results showed that human adipose tissue-derived stem cells maintained their morphological and biological properties during 10 passages and after performing further tests mainly addressed the cytogenetic stability and expression of regulators of cell cycle, senescence and apoptosis, they should be safely used in clinical practice.

OnLine Journal of Biological Sciences
Volume 16 No. 4, 2016, 145-151

DOI: https://doi.org/10.3844/ojbsci.2016.145.151

Submitted On: 3 June 2016 Published On: 17 December 2016

How to Cite: Oravcova, L., Bohac, M., Krajciova, L., Polak, S., Bohmer, D. & Danisovic, L. (2016). Effect of Serial Passaging on the Morphology and Biological Characteristics of Human Adipose Tissue-derived Stem Cells. OnLine Journal of Biological Sciences, 16(4), 145-151. https://doi.org/10.3844/ojbsci.2016.145.151

Open AccessCopyright: © 2016 Lenka Oravcova, Martin Bohac, Lubica Krajciova, Stefan Polak, Daniel Bohmer and Lubos Danisovic. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Keywords

  • Stem Cells
  • Adipose Tissue
  • Long Term Culture
  • Morphology
  • Biological Properties