Early Responses of Neural Cells (U87) to Particulate Matter Exposure: Cell Viability and Cytokine Concentration

Abstract

Particulate Matter (PM) has a significant impact on human health and increases the risk of respiratory diseases. PM exposure is linked to various illnesses, including cardiovascular diseases, cancer, neurological diseases, and pregnancy complications, and contributes to both mortality and morbidity globally. PM can also influence the autonomic nervous system. The evidence suggests that the likelihood of developing lung cancer continually rises with the rise in the concentration of coarse PM₁₀ (medium aerodynamic diameter less than or equal to 10 μm). However, studies examining the relationship between PM₁₀ exposure and brain cancer or gliomas are scarce. In this study, U87 cells were exposed to PM₁₀ at three different concentrations and the effects were evaluated by analyzing Lactate Dehydrogenase (LDH) activity, Interleukin-6 (IL-6) expression, and cell viability using a water-soluble tetrazolium assay. The IL-6 concentration in U87 cells varied with PM₁₀ concentration and exposure time. PM₁₀ did not influence LDH activity in U87 cells at the tested concentrations. These results provide crucial information for future research aimed at understanding the impact of PM on human disease pathology.