Research Article Open Access

Early Responses of Neural Cells (U87) to Particulate Matter Exposure: Cell Viability and Cytokine Concentration

Jae-Sik Jeon1, Eun Ju Oh2, Qian-Wen Wang1 and Jae Kyung Kim1
  • 1 Department of Biomedical Laboratory Science, Dankook University College of Health Sciences, Cheonan-si, Chungnam, Republic of Korea
  • 2 Department of Medical Laser, Dankook University Graduate School of Medicine, Cheonan-si, Chungnam, Republic of Korea

Abstract

Particulate Matter (PM) has a significant impact on human health and increases the risk of respiratory diseases. PM exposure is linked to various illnesses, including cardiovascular diseases, cancer, neurological diseases, and pregnancy complications, and contributes to both mortality and morbidity globally. PM can also influence the autonomic nervous system. The evidence suggests that the likelihood of developing lung cancer continually rises with the rise in the concentration of coarse PM10 (medium aerodynamic diameter less than or equal to 10 μm). However, studies examining the relationship between PM10 exposure and brain cancer or gliomas are scarce. In this study, U87 cells were exposed to PM10 at three different concentrations and the effects were evaluated by analyzing Lactate Dehydrogenase (LDH) activity, Interleukin-6 (IL-6) expression, and cell viability using a water-soluble tetrazolium assay. The IL-6 concentration in U87 cells varied with PM10 concentration and exposure time. PM10 did not influence LDH activity in U87 cells at the tested concentrations. These results provide crucial information for future research aimed at understanding the impact of PM on human disease pathology.

American Journal of Biochemistry and Biotechnology
Volume 19 No. 1, 2023, 65-70

DOI: https://doi.org/10.3844/ajbbsp.2023.65.70

Submitted On: 24 October 2022 Published On: 20 February 2023

How to Cite: Jeon, J., Oh, E. J., Wang, Q. & Kim, J. K. (2023). Early Responses of Neural Cells (U87) to Particulate Matter Exposure: Cell Viability and Cytokine Concentration. American Journal of Biochemistry and Biotechnology, 19(1), 65-70. https://doi.org/10.3844/ajbbsp.2023.65.70

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Keywords

  • Cell Viability
  • Cytotoxicity
  • IL-6
  • Particulate Matter
  • U87 Cells