Research Article Open Access

Extraction Process Optimization and Functional Characteristics of by Papain Solubilized Collagen from Large Yellow Croaker (Pseudosciaena crocea) Skin

Biaoshi Wang1, Wenfeng Li1, Shumin Liu1, Xiaojun Hu1, Shiqi Zhang1 and Shengyuan Yang1
  • 1 School of Food Science and Engineering, Lingnan Normal University, Zhanjiang, Guangdong, China

Abstract

Through collagen extraction from large yellow croaker (Pseudosciaena crocea) skin using an enzymatic method, the extraction optimization and characterization of collagen from raw materials after papain hydrolysis pretreatment are studied. The extraction process was optimized using the response surface methodology in this study. The findings show that the optimal conditions are a ratio (solvent to solid) of 25.0 mL/g, a time of 42.0 h, a temperature of 40.0°C and a pH of 7.0. Under these optimum conditions, the collagen extraction rate is 66.17%. Based on the FTIR spectra analysis, collagen can maintain a high-interacted structure, and collagen peptides have a complete triple helix structure. In addition, the SEM analysis shows that the extracted collagen has well-defined fibril morphologies. Also, the foaming capacity and emulsifying ability of extracted collagen are superior to commercial type I collagen.

American Journal of Biochemistry and Biotechnology
Volume 19 No. 4, 2023, 347-357

DOI: https://doi.org/10.3844/ajbbsp.2023.347.357

Submitted On: 24 July 2023 Published On: 26 December 2023

How to Cite: Wang, B., Li, W., Liu, S., Hu, X., Zhang, S. & Yang, S. (2023). Extraction Process Optimization and Functional Characteristics of by Papain Solubilized Collagen from Large Yellow Croaker (Pseudosciaena crocea) Skin. American Journal of Biochemistry and Biotechnology, 19(4), 347-357. https://doi.org/10.3844/ajbbsp.2023.347.357

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Keywords

  • Large Yellow Croaker Skin
  • Collagen
  • Extraction
  • Characteristics
  • Response Surface Methodology (RSM)