Extraction Process Optimization and Functional Characteristics of by Papain Solubilized Collagen from Large Yellow Croaker (Pseudosciaena crocea) Skin
- 1 School of Food Science and Engineering, Lingnan Normal University, Zhanjiang, Guangdong, China
Abstract
Through collagen extraction from large yellow croaker (Pseudosciaena crocea) skin using an enzymatic method, the extraction optimization and characterization of collagen from raw materials after papain hydrolysis pretreatment are studied. The extraction process was optimized using the response surface methodology in this study. The findings show that the optimal conditions are a ratio (solvent to solid) of 25.0 mL/g, a time of 42.0 h, a temperature of 40.0°C and a pH of 7.0. Under these optimum conditions, the collagen extraction rate is 66.17%. Based on the FTIR spectra analysis, collagen can maintain a high-interacted structure, and collagen peptides have a complete triple helix structure. In addition, the SEM analysis shows that the extracted collagen has well-defined fibril morphologies. Also, the foaming capacity and emulsifying ability of extracted collagen are superior to commercial type I collagen.
DOI: https://doi.org/10.3844/ajbbsp.2023.347.357
Copyright: © 2023 Biaoshi Wang, Wenfeng Li, Shumin Liu, Xiaojun Hu, Shiqi Zhang and Shengyuan Yang. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Large Yellow Croaker Skin
- Collagen
- Extraction
- Characteristics
- Response Surface Methodology (RSM)