Research Article Open Access

Antioxidant Activity of Trilobatin and Its Inhibitory Effects on Oxidative Damage of Biological Macromolecules

Yuhan Zhai1, Yuqing Zhang1, Yaping Li1, Ming He1, Shuyan Yu1, Shaoxuan Yu1, Haifang Xiao1 and Yuanda Song1
  • 1 Department of Food Science and Engineering, Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, 266 Xincun West Road, Zibo, China


Excessive levels of free radicals in the body cause oxidative damage to macromolecules by injuring DNA, proteins, and lipids, which leads to structural and functional defects and ultimately results in a number of illnesses. The antioxidation of trilobatin and its inhibitory activity of trilobatin on oxidative damage of biological macromolecules have not been thoroughly investigated. In this research, the effects of trilobatin on scavenging rates of hydroxyl l, 1,1-Diphenyl-2-Picrylhydrazyl (DPPH), and 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals as well as reducing power were thus examined. Meanwhile, Cu2+/ H2O2 and 2, 2'-Azobis (2-methylpropionamidine) dehydrochloride (AAPH) induced proteins, lipids and DNA models were used to examine the inhibitory effects of trilobatin on oxidative damage of biological macromolecules. Through the significance analysis of the repeated experiment results, it was found that trilobatin had significant scavenging effects on DPPH and ABTS free radicals, which were up to and (100.07±1.93%), respectively. However, trilobatin had a limited capacity to scavenge hydroxyl radicals and reduce power. Moreover, trilobatin had protective effects on proteins, lipids, and DNA. However, the protective effects were different in different induction systems. These findings indicated that trilobatin may function as a natural antioxidant to maintain the balance between oxidation and antioxidation in organisms. Theoretical support for the use of trilobatin in functional foods was offered by this research.

American Journal of Biochemistry and Biotechnology
Volume 19 No. 4, 2023, 282-291


Submitted On: 25 July 2023 Published On: 28 October 2023

How to Cite: Zhai, Y., Zhang, Y., Li, Y., He, M., Yu, S., Xiao, H. & Song, Y. (2023). Antioxidant Activity of Trilobatin and Its Inhibitory Effects on Oxidative Damage of Biological Macromolecules. American Journal of Biochemistry and Biotechnology, 19(4), 282-291.

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  • Trilobatin
  • Antioxidation
  • Biomacromolecule
  • Oxidative Damage