Responses of Neural Cells (U87) to HCoV-229E and HCoV-OC43 Infections
- 1 Department of Medical Laser, Dankook University Graduate School of Medicine, Cheonan-si, Chungnam, Republic of Korea
- 2 Department of Biomedical Laboratory Science, Dankook University College of Health Sciences, Cheonan-si, Chungnam, Republic of Korea
- 3 Department of Biomedical Laboratory Science, Dankook University College of Health Sciences, Cheonan-si, Chungnam, Republic of Korea
Abstract
Respiratory viruses, such as Coronaviruses (CoVs), can be neuroinvasive and exacerbate neurological pathologies via their capacity for direct viral replication and/or by inducing excessive host immune responses in the Central Nervous System (CNS). HCoV-229E is a primary COVID-19 and HCoV-OC43 is a beta COVID-19 belonging to the same genus as SARS-CoV-2 and both can cause acute infections in glioblastoma, neuroblastoma, and germline cells. These viruses tend to exhibit different infectivity mechanisms and HCoV-OC43 tends to be more toxic than HCoV-229E for CNS cells. To gain an in-depth understanding of how respiratory viruses, such as SARS-CoV, infect nerve cells (U87) and promote inflammation and determine the different mechanisms underlying HCoV-229E and HCoV-OC43 infection, we evaluated Lactic Acid Dehydrogenase (LDH) activity, cell viability, and IL-8 absorptions. HCoV-OC43 tended to show greater cytotoxicity against U87 nerve cells than HCoV-229E. Further study into the connection between the HCoV-229E and HCoV-OC43 viruses and brain cell reactions will be supported by our results.
DOI: https://doi.org/10.3844/ajbbsp.2023.169.174
Copyright: © 2023 Eun Ju Oh, Jae-Sik Jeon, Qian-Wen Wang and Jae Kyung Kim. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- Cell Viability
- Cytotoxicity
- HCoV-OC43
- HCoV-229E
- U87 Cells