Research Article Open Access

Responses of Neural Cells (U87) to HCoV-229E and HCoV-OC43 Infections

Eun Ju Oh1, Jae-Sik Jeon2, Qian-Wen Wang 2 and Jae Kyung Kim3
  • 1 Department of Medical Laser, Dankook University Graduate School of Medicine, Cheonan-si, Chungnam, Republic of Korea
  • 2 Department of Biomedical Laboratory Science, Dankook University College of Health Sciences, Cheonan-si, Chungnam, Republic of Korea
  • 3 Department of Biomedical Laboratory Science, Dankook University College of Health Sciences, Cheonan-si, Chungnam, Republic of Korea

Abstract

Respiratory viruses, such as Coronaviruses (CoVs), can be neuroinvasive and exacerbate neurological pathologies via their capacity for direct viral replication and/or by inducing excessive host immune responses in the Central Nervous System (CNS). HCoV-229E is a primary COVID-19 and HCoV-OC43 is a beta COVID-19 belonging to the same genus as SARS-CoV-2 and both can cause acute infections in glioblastoma, neuroblastoma, and germline cells. These viruses tend to exhibit different infectivity mechanisms and HCoV-OC43 tends to be more toxic than HCoV-229E for CNS cells. To gain an in-depth understanding of how respiratory viruses, such as SARS-CoV, infect nerve cells (U87) and promote inflammation and determine the different mechanisms underlying HCoV-229E and HCoV-OC43 infection, we evaluated Lactic Acid Dehydrogenase (LDH) activity, cell viability, and IL-8 absorptions. HCoV-OC43 tended to show greater cytotoxicity against U87 nerve cells than HCoV-229E. Further study into the connection between the HCoV-229E and HCoV-OC43 viruses and brain cell reactions will be supported by our results.

American Journal of Biochemistry and Biotechnology
Volume 19 No. 2, 2023, 169-174

DOI: https://doi.org/10.3844/ajbbsp.2023.169.174

Submitted On: 11 January 2023 Published On: 8 June 2023

How to Cite: Oh, E. J., Jeon, J., Wang , Q. & Kim, J. K. (2023). Responses of Neural Cells (U87) to HCoV-229E and HCoV-OC43 Infections. American Journal of Biochemistry and Biotechnology, 19(2), 169-174. https://doi.org/10.3844/ajbbsp.2023.169.174

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Keywords

  • Cell Viability
  • Cytotoxicity
  • HCoV-OC43
  • HCoV-229E
  • U87 Cells