Research Article Open Access

Screening of Dominant Cellulose-Degrading Microbe in Humus and Optimisation of its Enzyme Producing Conditions and Application Optimization of Enzyme Production Conditions and Evaluation of Efficient Straw Degradation by Penicillium oxalicum

Gailing Wang1, Chendi Li1, Yanan Guo2, Lei Guo3, Mingcheng Wang1, Tongbiao Li1, Linglong Xu 1 and Enzhong Li1
  • 1 School of Biological and Food Engineering, Huanghuai University, Zhumadian, Henan 463000, China
  • 2 Institute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, Ningxia, 750002, China
  • 3 Institute of Poultry Research, Ningxia Xiaoming Agriculture and Animal Husbandry Co., Ltd, China

Abstract

The slow degradation of returned straw adversely affects seedling survival rate. To shorten the degradation time of lignin in maize straw in warm temperate regions, a cellulolytic microbial strain that can secrete polymer with high enzymatic activity was isolated from the humus, which helped to identify an effective strain to expedite the decomposition of returned maize straw. Using the molecular biology techniques of primary screening and re-screening using selective media, the dominant cellulolytic microbes in the humus were identified as Penicillium oxalicum (P. oxalicum) (KY781806.1). Inoculum size, initial pH, fermentation time, and temperature were optimized to increase cellulase activity. The activities of sodium Carboxymethyl Cellulose (CMC) and Filter Paper (FPA) were increased by 23.04 and 25.10%, respectively. The ability of P. oxalicum to utilize cellulose was also demonstrated using a filter paper degradation test and maize straw utilization experiment. Finally, the results of a field test showed that the addition of P. oxalicum X5 caused the returned straw to degrade completely within 30 days.

American Journal of Biochemistry and Biotechnology
Volume 19 No. 2, 2023, 159-168

DOI: https://doi.org/10.3844/ajbbsp.2023.159.168

Submitted On: 14 February 2023 Published On: 23 May 2023

How to Cite: Wang, G., Li, C., Guo, Y., Guo, L., Wang, M., Li, T., Xu , L. & Li, E. (2023). Screening of Dominant Cellulose-Degrading Microbe in Humus and Optimisation of its Enzyme Producing Conditions and Application Optimization of Enzyme Production Conditions and Evaluation of Efficient Straw Degradation by Penicillium oxalicum. American Journal of Biochemistry and Biotechnology, 19(2), 159-168. https://doi.org/10.3844/ajbbsp.2023.159.168

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Keywords

  • Humus
  • Polymer
  • Cellulolytic Microbial Species
  • Purification
  • Function
  • Enzyme