Evaluation of Mir 29c-3p and Mir 31-5p Serum Values in Predicting Nephropathy in Type 2 Diabetic Patients
- 1 Menoufia University Faculty of Medicine, Egypt
Abstract
Diabetic Nephropathy (DN) is a prime complication of diabetes with various pathological mechanisms. microRNAs (miRNAs) have emerged as an important factor in the pathogenesis of renal disorders. We aimed to analyze serum miR 29c-3p and miR 31-5p in diabetic patients in relation to albuminuria and eGFR. Also, the correlations between these miRNAs and cystatin C were assessed. This study was conducted on 180 Type 2 Diabetes Mellitus (T2DM) patients and 60 age and gender matched controls. The patients were divided into three groups: Normoalbuminuric (Group I), microalbuminuric (Group II) and macroalbuminuric (Group III). miRNA expression analysis was assessed via real-time PCR. The diabetic patients showed decreased miR 29c-3p and miR 31-5p serum values and increased cystatin C compared with the controls. miR31-5p had better sensitivity (92.31%), specificity (93.62%) and AUC (0.958) than miR 29c-3p (69.23% sensitivity, 85.11% specificity and AUC = 0.785) in the prediction of an eGFR <60 mL/min/1.73 m2 in normoalbuminuric patients. Both miR 29c-3p and miR 31-5p could be used as early diagnostic and prognostic biomarkers for DN in T2DM. In particular, circulating miR 31-5p outperformed the other miRNAs in detecting normoalbuminuric diabetic patients with lower eGFRs.
DOI: https://doi.org/10.3844/ajbbsp.2022.68.77
Copyright: © 2022 Mona S. Habieb, Nesreen Gamal Elhelbawy, Khaled M. Elzorkany, Mohamed Z. Nooh, Mohammad G. Elhelbawy and Heba F. Khader. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Keywords
- miR 29c-3p
- miR 31-5p
- Cystatin C
- T2DM