The Effect of Plant Growth Regulators (PGRs) on Efficient Regeneration of 12 Recalcitrant Indica Rice (Oryza Sativa L.) Genotypes
- 1 Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Bangladesh
Biotechnological approaches provide an efficient and expeditious system for developing agronomic features of rice at the cellular and molecular level compared to traditional breeding methods. The in-vitro regeneration method optimization of recalcitrant indica rice genotypes is a critical step for the biotechnological approaches for further development as the optimum concentrations of Plant Growth Regulators (PGRs) for in-vitro regeneration vary from genotype to genotype. Here, 12 important rice genotypes, including local cultivars and varieties, were collected from different regions of Bangladesh and evaluated for their in-vitro regeneration potentiality. Mature dehusked sterilized rice seeds were inoculated on different basal media (MS, N6, B5) supplemented with different concentrations of 2,4-Dichlorophenoxyaceitic acid (2,4-D) for callus induction. Induced calli were transferred to N6 media supplemented with different combinations of 6-Benzylaminopurine (BA) and 1-Napthaleneacetic Acid (NAA) for shoot induction. Regenerated shoots were rooted on half-strength MS media without supplementation of any hormones. Treatments of different concentrations and combinations of plant growth regulators showed varying results. Maximum callus induction frequencies were observed with 2.5-3.0 mg/l supplementation of 2,4-D on N6 media where Saat, Jamaibabu and Nimikhanai showed the highest callus induction frequencies (84.44%; 78.89 and 80% respectively) and maximum shoot initiation was observed at 2.5 mg/l BA +1.0 mg/l NAA and 3.0 mg/l 6-BA +1.5 mg/l NAA supplemented media where rice genotypes BRRI Dhan71, Nayapaijam, Nimikhanai and BRRI Dhan28 showed the highest shoot induction frequencies-86.67, 85.00, 86.67 and 88.33% respectively. Nearly all of the induced shoots showed root formation on half-strength MS media. This study demonstrates variations in regeneration response of the rice genotypes where cultivars Jamaibabu, Nayapaijam and Nimikhanai showed the highest in-vitro regeneration potential.
Copyright: © 2022 Md. Nazmul Hasan, Fahmid H. Bhuiyan, Hammadul Hoque, Nurnabi Azad Jewel, Md. Ashrafuzzaman and Shamsul H. Prodhan. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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- Indica Rice