Research Article Open Access

Characterization, Antioxidant Activities and Optimization of Preparation Process of a Auricularia Polytricha Polysaccharide-Zinc (II) Complex

Xinyi Wu1, Xiao Ding1, Ze Zhang1, Ziyi Xu1, Jiehan Zhang1, Wen Zhao1 and Yiyong Chen1
  • 1 Changshu Institute of Technology, China

Abstract

In our previous work, a novel polysaccharide APP3a from Auricularia polytricha was successfully isolated and purified. Response surface methodology was used to optimize the preparation process of APP3a-Zn on the basis of the single factor experiments. Characterization of APP3a-Zn was carried out and its antioxidant activity was also investigated. The result showed that the best preparation process of APP3a-Zn was chelation time 4.45 h, chelation temperature 49.37°C and pH 4.68. The weight loss of APP3a-Zn was slightly smaller than that of APP3a within the heating temperature range and the crystal structure of APP3a-Zn had undergone tremendous changes compared with APP3a. APP3a-Zn had stronger antioxidant activity than APP3a. The results indicated that chelation with zinc enhanced the antioxidant activity of APP3a. In the food industry, APP3a-Zn had great potential as a new zinc supplement with antioxidant activity.

American Journal of Biochemistry and Biotechnology
Volume 17 No. 1, 2021, 118-129

DOI: https://doi.org/10.3844/ajbbsp.2021.118.129

Submitted On: 14 January 2021 Published On: 25 March 2021

How to Cite: Wu, X., Ding, X., Zhang, Z., Xu, Z., Zhang, J., Zhao, W. & Chen, Y. (2021). Characterization, Antioxidant Activities and Optimization of Preparation Process of a Auricularia Polytricha Polysaccharide-Zinc (II) Complex. American Journal of Biochemistry and Biotechnology, 17(1), 118-129. https://doi.org/10.3844/ajbbsp.2021.118.129

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Keywords

  • Auricularia polytricha
  • Zinc
  • Polysaccharide
  • Chelation
  • Characterization
  • Antioxidant Activities