Study on Carbon Source Screening Technology for Prevention and Control of Banana Fusarium Wilt

Abstract

With the spread of banana Fusarium wilt and the aggravation of the damage, it has become the biggest threat to the banana industry. Many studies have shown that bio-organic fertilizer plays an important role in the prevention and control of banana wilt, but most studies focused on biocontrol bacterium screening, not screening carbon sources that could prevent banana wilt, which lead to organic fertilizer fertilization without better effect. So that, a pot culture experiment (I) with inoculating Fusarium oxysporum f. sp. Cubense (FOC) on bananas was carried out to screen the carbon sources, and d-malic acid (CS2) to prevent and control banana wilt had been screened out by Biolog technology and Principal Component Analysis of microbial community level physiological profiling. To verify application effects of CS2, another banana pot experiment (II) with FOC inoculation had been done. The results showed that: (1) CS2 can effectively improve the activity of soil microorganisms. (2) CS2 can significantly reduce FOC population in soil, and the FOC population treated with CS2 was respectively lower than that treated with control carbon source (CS1) and without carbon source (CK1) 48% and 59%. (3) The banana wilt infection rate and disease index of CS2 treatment were significantly smaller than those of CS1 and CK1. (4) CS2 can promote the growth and biomass accumulation of bananas in pathogenic soil. In conclusion, the screening technology studied here can be applied to screen out the carbon sources for prevention of banana Fusarium wilt, which has great significance to the improvement of prevention effect of biological organic fertilizers to banana wilt.